Application of laser-induced breakdown spectroscopy to the analysis of algal biomass for industrial biotechnology

被引：26

作者：

Porizka, P. ^{[1
]}

Prochazka, D. ^{[1
]}

Pilat, Z. ^{[2
]}

Krajcarova, L. ^{[3
]}

Kaiser, J. ^{[1
]}

Malina, R. ^{[1
]}

Novotny, J. ^{[1
]}

Zemanek, P. ^{[2
]}

Jezek, J. ^{[2
]}

Sery, M. ^{[2
]}

Bernatova, S. ^{[2
]}

Krzyzanek, V. ^{[2
]}

Dobranska, K. ^{[2
]}

Novotny, K. ^{[3
]}

Trtilek, M. ^{[4
]}

Samek, O. ^{[2
]}

机构：

[1] Brno Univ Technol, CEITEC Cent European Inst Technol, Xray Micro CT & Nano CT Res Grp, Brno 61600, Czech Republic

[2] Acad Sci Czech Republ, Inst Sci Instruments, ASCR Vvi, Brno 61669, Czech Republic

[3] Masaryk Univ, Dept Chem, Fac Sci, CS-61137 Brno, Czech Republic

[4] Photon Syst Instruments, Drasov 66424, Czech Republic

来源：

SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY | 2012年 / 74-75卷

关键词：

LIBS; Double-pulse; Water-jet; Algal biomass; Biotechnology; WATER; STRONTIUM; ABLATION; CADMIUM; METALS; PLASMA;

D O I：

10.1016/j.sab.2012.06.014

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

We report on the application of laser-induced breakdown spectroscopy (LIBS) to the determination of elements distinctive in terms of their biological significance (such as potassium, magnesium, calcium, and sodium) and to the monitoring of accumulation of potentially toxic heavy metal ions in living microorganisms (algae); in order to trace e.g. the influence of environmental exposure and other cultivation and biological factors having an impact on them. Algae cells were suspended in liquid media or piesented in a form of adherent cell mass on a surface (biofilm) and, consequently, characterized using their spectra. In our feasibility study we used three different experimental arrangements employing double-pulse LIBS technique in order to improve on analytical selectivity and sensitivity for potential industrial biotechnology applications, e.g. for monitoring of mass production of commercial biofuels, utilization in the food industry and control of the removal of heavy metal ions from industrial waste waters. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：169 / 176

页数：8

共 28 条

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