Efficacy and molecular studies of a Lebanese isolate of Beauveria for control of Thaumetopoea wilkinsoni (Lepidoptera: Thaumetopoeidae)

被引：4

作者：

Abou-Jawdah, Yusuf ^{[1
]}

Atamian, Hagop ^{[1
]}

Nemer, Georges ^{[2
]}

Kfoury, Linda ^{[3
]}

Choukrallah, Nancy ^{[3
]}

Hanna, Lucia ^{[1
]}

Nemer, Nabil ^{[1
]}

机构：

[1] Amer Univ Beirut, Dept Agr Sci, Fac Agr & Food Sci, Beirut, Lebanon

[2] Amer Univ Beirut, Dept Biochem, Beirut, Lebanon

[3] Lebanese Univ, Fac Agr, Beirut, Lebanon

来源：

BIOCONTROL SCIENCE AND TECHNOLOGY | 2008年 / 18卷 / 06期

关键词：

Beauveria; Thaumetopoea wilkinsoni; pine processionary moth; DNA lyase gene;

D O I：

10.1080/09583150802090018

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A Lebanese isolate of a Beauveria species originally isolated from a hymenopterous insect pest was found to be very effective against the pine processionary moth Thaumetopoea wilkinsoni. In laboratory bioassays, high mortality rates of the five larval stages were attained with spore concentrations of about 5000-50,000 spores/larva. The efficacy of kill on the first three larval stages was similar to the growth regulator diflubenzuron, but it was significantly higher on the fourth and fifth larval stages. Sequences of the DNA lyase gene and the EF-1 alpha gene were used for molecular characterisation of this Beauveria isolate. The DNA lyase gene showed more polymorphism than the previously reported ITS region and EF-1 alpha gene. This constitutes the first report on the possibility of using the DNA lyase gene as a molecular tool in fungal taxonomy.

引用

页码：581 / 589

页数：9

共 21 条

[1] Isolation of Beauveria species from Lebanon and evaluation of its efficacy against the cedar web-spinning sawfly, Cephalcia tannourinensis [J].

Abdo, Carla ;

Nemer, Nabil ;

Nemer, Georges ;

Abou Jawdah, Yusuf ;

Atamian, Hagop ;

Kawar, Nasri S. .

BIOCONTROL, 2008, 53 (02) :341-352

[2]

ALTSCHUL SF, 1997, NUCLEIC ACIDS RES, V25, P3402

[3] Arthropod allergens and human health [J].

Arlian, LG .

ANNUAL REVIEW OF ENTOMOLOGY, 2002, 47 :395-433

[4] Predation by the hoopoe (Upupa epops) on pupae of Thaumetopoea pityocampa and the likely influence on other natural enemies [J].

Battisti, A ;

Bernardi, M ;

Ghiraldo, C .

BIOCONTROL, 2000, 45 (03) :311-323

[5] PRODUCTION, FORMULATION AND APPLICATION OF THE ENTOMOPATHOGENIC FUNGUS BEAUVERIA-BASSIANA FOR INSECT CONTROL - CURRENT STATUS [J].

FENG, MG ;

POPRAWSKI, TJ ;

KHACHATOURIANS, GG .

BIOCONTROL SCIENCE AND TECHNOLOGY, 1994, 4 (01) :3-34

[6] Morphological and genetic characterisation of Beauveria spp. from New Zealand [J].

Glare, TR ;

Inwood, AJ .

MYCOLOGICAL RESEARCH, 1998, 102 :250-256

[7] Bacillus thuringiensis Cry1Ca-resistant Spodoptera exigua lacks expression of one of four Aminopeptidase N genes -: art. no. 96 [J].

Herrero, S ;

Gechev, T ;

Bakker, PL ;

Moar, WJ ;

de Maagd, RA .

BMC GENOMICS, 2005, 6 (1)

[8] The potential of Beauveria bassiana (Hyphomycetes: Moniliales) as a biological control agent against the pine beauty moth, Panolis flammea (Lepidoptera: Noctuidae) [J].

Hicks, BJ ;

Watt, AD ;

Cosens, D .

FOREST ECOLOGY AND MANAGEMENT, 2001, 149 (1-3) :275-281

[9] Reconstructing the early evolution of Fungi using a six-gene phylogeny [J].

James, Timothy Y. ;

Kauff, Frank ;

Schoch, Conrad L. ;

Matheny, P. Brandon ;

Hofstetter, Valerie ;

Cox, Cymon J. ;

Celio, Gail ;

Gueidan, Cecile ;

Fraker, Emily ;

Miadlikowska, Jolanta ;

Lumbsch, H. Thorsten ;

Rauhut, Alexandra ;

Reeb, Valerie ;

Arnold, A. Elizabeth ;

Amtoft, Anja ;

Stajich, Jason E. ;

Hosaka, Kentaro ;

Sung, Gi-Ho ;

Johnson, Desiree ;

O'Rourke, Ben ;

Crockett, Michael ;

Binder, Manfred ;

Curtis, Judd M. ;

Slot, Jason C. ;

Wang, Zheng ;

Wilson, Andrew W. ;

Schuessler, Arthur ;

Longcore, Joyce E. ;

O'Donnell, Kerry ;

Mozley-Standridge, Sharon ;

Porter, David ;

Letcher, Peter M. ;

Powell, Martha J. ;

Taylor, John W. ;

White, Merlin M. ;

Griffith, Gareth W. ;

Davies, David R. ;

Humber, Richard A. ;

Morton, Joseph B. ;

Sugiyama, Junta ;

Rossman, Amy Y. ;

Rogers, Jack D. ;

Pfister, Don H. ;

Hewitt, David ;

Hansen, Karen ;

Hambleton, Sarah ;

Shoemaker, Robert A. ;

Kohlmeyer, Jan ;

Volkmann-Kohlmeyer, Brigitte ;

Spotts, Robert A. .

NATURE, 2006, 443 (7113) :818-822

[10]

Larone DH., 1995, Medically Important Fungi-A Guide to Identification, V3rd edn.

← 1 2 3 →