Functional analysis of the YvrGHb two-component system of Bacillus subtilis:: Identification of the regulated genes by DNA microarray and northern blot analyses

被引：41
作者：
Serizawa, M
Kodama, K
Yamamoto, H
Kobayashi, K
Ogasawara, N
Sekiguchi, J
机构：
[1] Shinshu Univ, Fac Text Sci & Technol, Dept Appl Biol, Ueda, Nagano 3868567, Japan
[2] Nara Inst Sci & Technol, Grad Sch Informat Sci, Nara 6300101, Japan
[3] Shinshu Univ, Div Gene Res, Res Ctr Human Environm Sci, Ueda, Nagano 3868567, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2005年 / 69卷 / 11期
基金：
日本学术振兴会;
关键词：
Bacillus subtilis; two-component system; cell surface proteins; extracytoplasmic function sigma factor (ECF sigma factor); antibiotic susceptibility;
D O I：
10.1271/bbb.69.2155
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
YvrGHb is a novel two-component system in Bacillus subtilis. The yvrG and yvrHb genes are considered to encode sensor kinase and response regulator respectively. We found that the YvrGHb system positively regulates the 7 transcriptional units (wprA, wapA-yxxG, dltABCDE, sunA, sun T-bdbA-yolJ-bdbB, yvrI-yvrHa, and sigX-rsiX), and negatively regulates the lytABC operon. wprA, wapA, lytB, and lytC encode the main cell surface proteins of B. subtilis. Furthermore, SigX [extracytoplasmic function sigma factor (ECF sigma factor)] relating to the cell surface homeostatic functions, was regulated by this system. The yvrGHb null mutant showed the unusual autolysis and higher susceptibility to the four kinds of antibiotics (aztreonam, cefepime, bacitracin, and fosfomycin). These results indicate that the YvrGHb system is important to maintain the overall state of the cell surface through transcriptional regulation.
引用
收藏
页码：2155 / 2169
页数：15
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