Platelet-derived growth factor BB stimulates differentiation of rat immature Leydig cells

被引：11

作者：

Wang, Yiyan ^{[1
,2
]}

Li, Xiaoheng ^{[1
,2
]}

Ge, Fei ^{[1
,2
]}

Yuan, Kaiming ^{[1
,2
]}

Su, Zhijian ^{[3
]}

Wang, Guimin ^{[1
,2
]}

Lian, Qingquan ^{[1
,2
]}

Ge, Ren-Shan ^{[1
,2
]}

机构：

[1] Wenzhou Med Univ, Affiliated Hosp 2, Dept Anesthesiol, Wenzhou, Peoples R China

[2] Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou, Peoples R China

[3] Jinan Univ, Inst Life & Hlth Engn, Guangzhou, Guangdong, Peoples R China

来源：

JOURNAL OF MOLECULAR ENDOCRINOLOGY | 2018年 / 60卷 / 01期

关键词：

Platelet-derived growth factor BB; rat immature Leydig cells; steroidogenic acute regulatory protein; Fos; REGULATORY PROTEIN GENE; TESTICULAR DEVELOPMENT; 2; POPULATIONS; RECEPTORS; TESTIS; IDENTIFICATION; DEHYDROGENASE; EXPRESSION; ANDROGEN; PROLIFERATION;

D O I：

10.1530/JME-17-0222

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Platelet-derived growth factor (PDGF) is one family of growth factors that regulate cell growth and differentiation. Rat Leydig cells express PDGF-beta receptor (PDGFRB) during pubertal development. However, the mechanism of PDGF in the regulation of Leydig cell development is unclear. In the present study, rat immature Leydig cells were isolated from the testes of 35-day-old Sprague-Dawley rats and treated with 1 and 10 ng/mL of PDGF-BB. After 24 h of treatment, these cells were harvested for genomics profiling and the medium steroids were measured. 1 and 10 ng/mL PDGF-BB significantly increased androgen production by rat immature Leydig cells. Genomics profiling analysis showed that the expression levels of steroidogenic acute regulatory protein (Star) were increased by 2-fold. Further analysis showed that Fos expression level was increased 2-and 5-fold by 1 and 10 ng/mL PDGF-BB, respectively. In conclusion, PDGF-BB stimulated the differentiation of rat immature Leydig cells via regulating Star.

引用

页码：29 / 43

页数：15

共 46 条

[1] Role of Platelet-Derived Growth Factors in the Testis [J].

Basciani, Sabrina ;

Mariani, Stefania ;

Spera, Giovanni ;

Gnessi, Lucio .

ENDOCRINE REVIEWS, 2010, 31 (06) :916-939

[2] Transcription of platelet-derived growth factor receptor α in Leydig cells involves specificity protein 1 and 3 [J].

Bergeron, Francis ;

Bagu, Edward T. ;

Tremblay, Jacques J. .

JOURNAL OF MOLECULAR ENDOCRINOLOGY, 2011, 46 (02) :125-138

[3] Developmental roles of platelet-derived growth factors [J].

Betsholtz, C ;

Karlsson, L ;

Lindahl, P .

BIOESSAYS, 2001, 23 (06) :494-507

[4] Pdgfr-α mediates testis cord organization and fetal Leydig cell development in the XY gonad [J].

Brennan, J ;

Tilmann, C ;

Capel, B .

GENES & DEVELOPMENT, 2003, 17 (06) :800-810

[5] Leydig cells: From stem cells to aging [J].

Chen, Haolin ;

Ge, Ren-Shan ;

Zirkin, Barry R. .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2009, 306 (1-2) :9-16

[6] Cyclic adenosine 3′,5′-monophosphate (cAMP) enhances cAMP-responsive element binding (CREB) protein phosphorylation and phospho-CREB interaction with the mouse steroidogenic acute regulatory protein gene promoter [J].

Clem, BF ;

Hudson, EA ;

Clark, BJ .

ENDOCRINOLOGY, 2005, 146 (03) :1348-1356

[7]

Cochran RC, 1981, INVESTIGATIVE UROLOG, V19

[8] Determination of the sensitising activity of the rubber contact sensitisers TMTD, ZDMC, MBT and DEA in a modified local lymph node assay and the effect of sodium dodecyl sulfate pretreatment on local lymph node responses [J].

De Jong, WH ;

Tentij, M ;

Spiekstra, SW ;

Vandebriel, RJ ;

Van Loveren, H .

TOXICOLOGY, 2002, 176 (1-2) :123-134

[9] Endocrine Disrupting Effects of Triclosan on the Placenta in Pregnant Rats [J].

Feng, Yixing ;

Zhang, Pin ;

Zhang, Zhaobin ;

Shi, Jiachen ;

Jiao, Zhihao ;

Shao, Bing .

PLOS ONE, 2016, 11 (05)

[10]

Ge R., 2007, LEYDIG CELL HLTH DIS, P55, DOI DOI 10.1007/978-1-59745-453-7_4

← 1 2 3 4 5 →