Optimizing sample handling for urinary proteomics

被引：54

作者：

Lee, Richard S. ^{[1
,2
]}

Monigatti, Flavio ^{[3
]}

Briscoe, Andrew C. ^{[2
]}

Waldon, Zachary ^{[3
]}

Freernan, Michael R. ^{[2
]}

Steen, Hanno ^{[2
,3
]}

机构：

[1] Childrens Hosp, Dept Urol, Prot Ctr, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Dept Urol, Urol Dis Res Ctr, Boston, MA 02115 USA

[3] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2008年 / 7卷 / 09期

基金：

瑞士国家科学基金会;

关键词：

biomarker; sample preparation; protein precipitation; ultrafiltration; mass spectrometry;

D O I：

10.1021/pr800301h

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Interrogation of the urinary proteome for clinically useful biomarkers of disease will require normalization of methods for protein extraction and sample handling. Variations in collection methods and other procedures may introduce significant discrepancies in qualitative and quantitative measurements. Here we demonstrate that the method of protein extraction, length of handling at room temperature, and repetitive freeze-thaw cycles do not seem to alter the urinary proteome at either the protein or peptide level in a manner that degrades information obtainable by mass spectrometry.

引用

页码：4022 / 4030

页数：9

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