Detection of chloramphenicol in meat with a chemiluminescence resonance energy transfer platform based on molecularly imprinted graphene

被引:43
|
作者
Jia, Bing Jie [1 ]
He, Xin [1 ]
Cui, Peng Lei [2 ]
Liu, Ju Xiang [1 ]
Wang, Jian Ping [1 ]
机构
[1] Hebei Agr Univ, Coll Vet Med, Baoding 071000, Hebei, Peoples R China
[2] Hebei Agr Univ, Coll Sci, Baoding 071000, Hebei, Peoples R China
关键词
Chloramphenicol; Molecularly imprinted microsphere; Magnetic graphene; Chemiluminescence resonance energy transfer; Meat; SOLID-PHASE EXTRACTION; SELECTIVE DETERMINATION; MILK SAMPLES; IMMUNOASSAY; POLYMER; SENSOR; PRECONCENTRATION; FLORFENICOL; PERFORMANCE; DISPERSION;
D O I
10.1016/j.aca.2019.02.044
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In this study, a novel composite was synthesized by polymerizing the dummy-template molecularly imprinted microspheres on the surface of magnetic graphene. This composite was used as recognition reagent and energy acceptor to develop a platform for determination of chloramphenicol according to the principle of chemiluminescence resonance energy transfer. The light signal was induced with luminol-H2O2-4-(imidazole-1-yl) phenol system, and the chemiluminescence intensity was positively correlated with the analyte concentration. The limit of detection for chloramphenicol in meat sample was 2.0 pg/g, and the recoveries from the standard fortified blank meat sample were in the range of 69.5%-97.3%. Furthermore, one single assay could be finished within 10 min, and the magnetic composite could be reused for at least thirty times. Therefore, this platform could be used as a rapid, simple, sensitive, accurate and recyclable tool for screening the residue of chloramphenicol in meat. (C) 2019 Elsevier B.V. All rights reserved.
引用
收藏
页码:136 / 143
页数:8
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