Potential of Cell-Free Supernatant from Lactobacillus plantarum NIBR97, Including Novel Bacteriocins, as a Natural Alternative to Chemical Disinfectants

被引:28
作者
Kim, Sam Woong [1 ]
Kang, Song I. [1 ]
Shin, Da Hye [1 ]
Oh, Se Yun [1 ]
Lee, Chae Won [2 ]
Yang, Yoonyong [2 ]
Son, Youn Kyoung [2 ]
Yang, Hee-Sun [2 ]
Lee, Byoung-Hee [2 ]
An, Hee-Jung [3 ]
Jeong, In Sil [4 ]
Bang, Woo Young [2 ]
机构
[1] Gyeongnam Natl Univ Sci & Technol, Gene Anal Ctr, Jinju 52725, South Korea
[2] Natl Inst Biol Resources NIBR, Environm Res Complex, Incheon 22689, South Korea
[3] CHA Univ, Dept Pathol, CHA Bundang Med Ctr, Seongnam 13496, South Korea
[4] CHA Adv Res Inst, Ctr Immune Cell Res, Seongnam 13488, South Korea
基金
新加坡国家研究基金会;
关键词
AMP; antimicrobial activity; antiviral activity; bacteriocin; COVID-19; disinfectant; Lactobacillus plantarum; plantaricin; ANTIMICROBIAL ACTIVITY; ANNOTATION; GENES;
D O I
10.3390/ph13100266
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
The recent pandemic of coronavirus disease 2019 (COVID-19) has increased demand for chemical disinfectants, which can be potentially hazardous to users. Here, we suggest that the cell-free supernatant from Lactobacillus plantarum NIBR97, including novel bacteriocins, has potential as a natural alternative to chemical disinfectants. It exhibits significant antibacterial activities against a broad range of pathogens, and was observed by scanning electron microscopy (SEM) to cause cellular lysis through pore formation in bacterial membranes, implying that its antibacterial activity may be mediated by peptides or proteins and supported by proteinase K treatment. It also showed significant antiviral activities against HIV-based lentivirus and influenza A/H3N2, causing lentiviral lysis through envelope collapse. Furthermore, whole-genome sequencing revealed that NIBR97 has diverse antimicrobial peptides, and among them are five novel bacteriocins, designated as plantaricin 1 to 5. Plantaricin 3 and 5 in particular showed both antibacterial and antiviral activities. SEM revealed that plantaricin 3 causes direct damage to both bacterial membranes and viral envelopes, while plantaricin 5 damaged only bacterial membranes, implying different antiviral mechanisms. Our data suggest that the cell-free supernatant from L. plantarum NIBR97, including novel bacteriocins, is potentially useful as a natural alternative to chemical disinfectants.
引用
收藏
页码:1 / 13
页数:13
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