Analysis and Preparation of Oligonucletide Microarray of Apoptosis-related Genes

With some conditions, there are 1 384 genes selected from 5 333 possible apoptosis sequences which were mined from IPI and GenBank. After the analysis of their subcellular location, tissue expression remarkably, natural antisense transcripts predict, gene clusters distributed in pathways, protein protein network, some interested things were mined. Some genes are differentially expressed in a tissue type; Some genes are NATS and some gene clusters are in more than one pathway. Meanwhile, one 40-bp oligonucleotide microarray which includes most apoptosis genes was made. With the microarray and samples of HeLaT-NAIF1 and HeLaT-pcDNA3, there were 24 genes differentialy expressed. Perhaps, when the Naif1 was over expressed. PAX2, PDCD8, PACD10, DFFA, CASP7 were also expressed differentially. And there is one mRNA, U58668, neither any gene nor protein information annotation, upregulated during camptothecin-induced apoptosis of U937 cells, also upregulated when the NAIF1 induced HeLaT cell apoptosis(all data is public in http://gpcrome.cbi.pku.edu.cn:2005/chip).