Characterization of hydrocarbon-degrading bacterial strains isolated from oil-polluted soil

被引：31

作者：

Tanase, Ana-Maria ^{[1
]}

Ionescu, Robertina ^{[1
]}

Chiciudean, Iulia ^{[1
]}

Vassu, Tatiana ^{[1
]}

Stoica, Ileana ^{[1
]}

机构：

[1] Univ Bucharest, Dept Genet, Sector 6, Bucharest 060101, Romania

来源：

INTERNATIONAL BIODETERIORATION & BIODEGRADATION | 2013年 / 84卷

关键词：

Biolog; 16S rDNA; n-hexadecane; n-alkane-hydroxylase; alkB; Achromobacter xylosoxidans; ALKANE HYDROXYLASE GENES; GRAM-POSITIVE STRAINS; PHYLOGENETIC ANALYSIS; ACINETOBACTER SP; RHODOCOCCUS SP; ALKB GENES; N-ALKANES; DEGRADATION; BIOREMEDIATION; ENVIRONMENT;

D O I：

10.1016/j.ibiod.2012.05.022

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Using enrichment culturing method, a microbial population was detected in an oil-contaminated soil nearby an extraction field. Isolated strains were able use medium-length n-alkanes as sole carbon and energy source as assessed by growth experiments. Results showed a high diversity among strains at molecular level, and also in the metabolic profiles. Physiological and biochemical tests showed a similarity within a group of four strains, as confirmed by Biolog MicroLog analysis. Based on 16S rDNA sequences strains were identified as Rhodococcus erythropolis, Acinetobacter baumanii, Burkholderia cepacia and Achromobacter xylosoxidans. Alkane monooxygenase gene (alkB) was successfully detected in all our strains and for the first time alkB genes were found in an A. xylosoxidans strain. This bacterial species has been previously reported as part of microbial communities from oil polluted environments, but there are few studies that address mechanisms details of A. xylosoxidans involvement in n-alkane degradation process. (c) 2012 Elsevier Ltd. All rights reserved.

引用

页码：150 / 154

页数：5

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