A modified aortic multiple-ring preparation for functional studies

A comparison is made of four rat aortic preparations, that is, single ring, spiral strip, zig-zag strip, and multiple-ring, on the basis of the responses to norepinephrine (NE) and acetylcholine (ACh). The single ring preparation was suitable under isometric, but not under isotonic conditions, because of the small isotonic tension which developed in response to the contractile agonist. The spiral and the zig-zag strips showed a discontinuity in the relaxant activity of the ACh, probably because of the removal, or not, of the endothelium from the preparation. The reproducibility of both contracturant and relaxing responses in the multiple-ring preparation makes this the most suitable of all four for the study of vasoactive drugs.