Identification and purity control of thioacridine derivatives by gas and capillary liquid chromatography with mass spectrometric detection

Mass spectrometry combined with gas chromatography (GC-MS) or capillary liquid chromatography (CLC-MS) was successfully utilized for structural characterization and purity control of synthesized biologically active thioacridine derivatives. Obtained; results document sufficient selectivity of GC-MS and CLC-MS towards structurally related impurities and degradation products accompanying the compound of desire in analyzed substances. Based on electron impact ionization mass spectra an attempt to estimate their exact structures was made. The results of structure elucidation were confirmed by more detailed fragmentation studies based on (MS)(2) and (MS)(3) electrospray ionization mass spectra.