The effect of polyvinylpyrrolidone (PVP) on the heavy chain monoclonal antibody production from plant suspension cultures

被引：49
作者：
LaCount, W
An, GH
Lee, JM
机构：
[1] WASHINGTON STATE UNIV,DEPT CHEM ENGN,PULLMAN,WA 99164
[2] WASHINGTON STATE UNIV,INST BIOL CHEM,PULLMAN,WA 99164
来源：
BIOTECHNOLOGY LETTERS | 1997年 / 19卷 / 01期
基金：
美国国家科学基金会;
关键词：
D O I：
10.1023/A:1018383524389
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A heavy chain monoclonal antibody (HC MAb) was produced by the suspension cultures of genetically modified tobacco cells. Though the HC MAb was secreted to the culture medium by the cells, it was unstable in plant cell media. The addition (0.75 g/L) of polyvinylpyrrolidone (MW 360,000) to the medium stabilized the extracellular HC MAb and increased its production 35 fold to 350 mu g/l.
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页码：93 / 96
页数：4
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