In situ isobaric lipid mapping by MALDI-ion mobility separation-mass spectrometry imaging

被引：37

作者：

Fu, Tingting ^{[1
]}

Oetjen, Janina ^{[2
]}

Chapelle, Manuel ^{[2
]}

Verdu, Alexandre ^{[2
]}

Szesny, Matthias ^{[2
]}

Chaumot, Arnaud ^{[3
]}

Degli-Esposti, Davide ^{[3
]}

Geffard, Olivier ^{[3
]}

Clement, Yohann ^{[1
]}

Salvador, Arnaud ^{[1
]}

Ayciriex, Sophie ^{[1
]}

机构：

[1] Univ Claude Bernard Lyon 1, Univ Lyon, Inst Sci Analyt, CNRS,UMR 5280, 5 Rue Doua, F-5280 Villeurbanne, France

[2] Bruker Daltonik GmbH, Bremen, Germany

[3] INRAE, UR RiverLy, Lab Ecotoxicol, Villeurbanne, France

来源：

JOURNAL OF MASS SPECTROMETRY | 2020年 / 55卷 / 09期

关键词：

ion mobility separation; lipid isomers; MALDI; mass spectrometry imaging; TIMS;

D O I：

10.1002/jms.4531

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The highly diverse chemical structures of lipids make their analysis directly from biological tissue sections extremely challenging. Here, we report the in situ mapping and identification of lipids in a freshwater crustaceanGammarus fossarumusing matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) in combination with an additional separation dimension using ion mobility spectrometry (IMS). The high-resolution trapped ion mobility spectrometry (TIMS) allowed efficient separation of isobaric/isomeric lipids showing distinct spatial distributions. The structures of the lipids were further characterized by MS/MS analysis. It is demonstrated that MALDI MSI with mobility separation is a powerful tool for distinguishing and localizing isobaric/isomeric lipids.

引用

页数：6

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