Structure of the O-polysaccharide of Providencia alcalifaciens O8 containing (2S,4R)-2,4-dihydroxypentanoic acid, a new non-sugar component of bacterial glycans

被引：9

作者：

Toukach, Filip V. ^{[1
]}

Kocharova, Nina A. ^{[1
]}

Maszewska, Agnieszka ^{[2
]}

Shashlov, Alexander S. ^{[1
]}

Knirel, Yuriy A. ^{[1
]}

Rozalski, Antoni ^{[2
]}

机构：

[1] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 119991, Russia

[2] Univ Lodz, Inst Microbiol & Immunol, PL-90237 Lodz, Poland

来源：

CARBOHYDRATE RESEARCH | 2008年 / 343卷 / 15期

关键词：

Providencia alcalifaciens; Lipopolysaccharide; O-Antigen; Bacterial polysaccharide structure; 2,4-Dihydroxypentanoic acid;

D O I：

10.1016/j.carres.2008.08.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A glycerol teichoic acid-like O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O8 and studied by chemical methods and NMR spectroscopy, including 2D ROESY, [H-1,C-13) HSQC, and HMQC-TOCSY experiments. It was found that the compound contains a new component of bacterial lipopolysaccharides: ether-linked (2S,4R)-2,4-dihydroxypentanoic acid (Dhpa), which was identified by NMR spectroscopy. The following structure of the repeating unit of the polysaccharide was established: [GRAPHICS] (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：2706 / 2711

页数：6

共 4 条

[1] Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O31 containing an ether Of D-mannose with (2R,4R)-2,4-dihydroxypentanoic acid
Ovchinnikova, Olga G.
Kocharova, Nina A.
Shashkov, Alexander S.
Bialczak-Kokot, Magdalena
Knirel, Yuriy A.
Rozalski, Antoni
CARBOHYDRATE RESEARCH, 2009, 344 (05) : 683 - 686
[2] Structure elucidation and gene cluster characterization of the O-antigen of Vibrio cholerae O68 containing (2S,4R)-2,4-dihydroxypentanoic acid
Li, Xiaodan
Perepelov, Andrei, V
Filatov, Andrei, V
Shashkov, Alexander S.
Liu, Bin
CARBOHYDRATE RESEARCH, 2019, 484
[3] The structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O36 containing 3-deoxy-D-manno-oct-2-ulosonic acid
Kocharova, Nina A.
Ovchinnikova, Olga G.
Torzewska, Agnieszka
Shashkov, Alexander S.
Knirel, Yuriy A.
Rozalski, Antoni
CARBOHYDRATE RESEARCH, 2007, 342 (3-4) : 665 - 670
[4] Structure of the O-antigen of Providencia stuartii O20, a new polysaccharide containing 5,7-diacetamido3,5,7,9-tetradeoxy-L-glycero-D-galacto-non-2-ulosonic acid
Shashkov, Alexander S.
Kocharova, Nina A.
Zatonsky, George V.
Blaszczyk, Aleksandra
Knirel, Yuriy A.
Rozalski, Antoni
CARBOHYDRATE RESEARCH, 2007, 342 (3-4) : 653 - 658

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