Lead Affects Vitamin D Metabolism in Rats

被引:26
作者
Rahman, Abdur [1 ]
Al-Awadi, Ameena A. [1 ]
Khan, Khalid M. [2 ]
机构
[1] Kuwait Univ, Coll Life Sci, Dept Food Sci & Nutr, POB 5969, Safat 13060, Kuwait
[2] Kuwait Univ, Fac Med, Dept Anat, POB 24923, Safat 13110, Kuwait
关键词
lead; vitamin D; 25-hydroxy vitamin D; 1,25-dihydroxy vitamin D; 25-hydroxylase; 1-alpha-hydroxylase; vitamin D receptor; rats; CALCITROPIC HORMONES; BONE TURNOVER; D-RECEPTOR; EXPOSURE; ASSOCIATION; MEMORY; BRAIN; VDR; NEUROTOXICITY; DEFICIENCY;
D O I
10.3390/nu10030264
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
A negative association between blood lead and vitamin D concentrations has been reported, however, experimental data on the effect of lead (Pb) on vitamin D metabolism is scarce. We investigated the effects of Pb on serum vitamin D metabolites, vitamin D activating enzymes and vitamin D receptor (VDR) in rats. NewbornWistar rat pups were exposed to 0.2% Pb-acetate via their dams' drinking water from post-natal day (PND) 1 to 21 and directly in drinking water until PND30. Serum 25-hydroxyvitamin D was analyzed with LC-MS/MS and 1,25-dihydroxyvitamin D with an immunoassay. Tissue expression of vitamin D activating enzymes and VDR were measured by Western blot and immunohistochemistry. Serum 25-hydroxyvitamin D was significantly decreased at both PND21 and PND30, whereas 1,25-dihydroxyvitamin D was decreased (p < 0.05) only at PND21 in the Pb-exposed rats. Expression of renal 1-alpha-hydroxylase was decreased by Pb only at PND21 (p < 0.05) but the brain 1-alpha-hydroxylase was not affected. Hepatic 25-hydroxylase expression was significantly decreased at PND21 but significantly increased at PND30 by Pb exposure. VDR expression in the brain was increased at both PND21 and PND30 (p < 0.05). These results suggest that Pb interferes with vitamin D metabolism by affecting the expression of its metabolizing enzymes.
引用
收藏
页数:17
相关论文
共 67 条
[51]  
Patrick L, 2006, ALTERN MED REV, V11, P2
[52]   Genetic modification of ALAD and VDR on lead-induced impairment of hearing in children [J].
Pawlas, Natalia ;
Broberg, Karin ;
Olewinska, Elzbieta ;
Kozlowska, Agnieszka ;
Skerfving, Staffan ;
Pawlas, Krystyna .
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 2015, 39 (03) :1091-1098
[53]   Modification by the genes ALAD and VDR of lead-induced cognitive effects in children [J].
Pawlas, Natalia ;
Broberg, Karin ;
Olewinska, Elzbieta ;
Prokopowicz, Adam ;
Skerfving, Staffan ;
Pawlas, Krystyna .
NEUROTOXICOLOGY, 2012, 33 (01) :37-43
[54]   Calcitropic hormones, bone turnover, and lead exposure among female smelter workers [J].
Potula, Vijayalakshmi ;
Henderson, Alden ;
Kaye, Wendy .
ARCHIVES OF ENVIRONMENTAL & OCCUPATIONAL HEALTH, 2005, 60 (04) :195-204
[55]   Over activation of hippocampal serine/threonine protein phosphatases PP1 and PP2A is involved in lead-induced deficits in learning and memory in young rats [J].
Rahman, Abdur ;
Khan, Khalid M. ;
Al-Khaledi, Ghanim ;
Khan, Islam ;
Al-Shemary, Tahany .
NEUROTOXICOLOGY, 2012, 33 (03) :370-383
[56]   Association of Maternal Blood Lead Level During Pregnancy with Child Blood Lead Level and Pregnancy Outcome in Kuwait [J].
Rahman, Abdur ;
Al-Rashidi, Haila A. G. ;
Khan, Abdul-Rehman .
ECOLOGY OF FOOD AND NUTRITION, 2012, 51 (01) :40-57
[57]   REDUCTION IN 1,25-DIHYDROXYVITAMIN D IN CHILDREN WITH INCREASED LEAD ABSORPTION [J].
ROSEN, JF ;
CHESNEY, RW ;
HAMSTRA, A ;
DELUCA, HF ;
MAHAFFEY, KR .
NEW ENGLAND JOURNAL OF MEDICINE, 1980, 302 (20) :1128-1131
[58]  
Schwalfenberg G.K., 2015, ScientificWorldJournal, V2015, P318595, DOI DOI 10.1155/2015/318595
[59]   EFFECT OF LEAD INGESTION ON FUNCTIONS OF VITAMIN-D AND ITS METABOLITES [J].
SMITH, CM ;
DELUCA, HF ;
TANAKA, Y ;
MAHAFFEY, KR .
JOURNAL OF NUTRITION, 1981, 111 (08) :1321-1329
[60]   Upregulation of vitamin D receptor levels by 1,25(OH)(2) vitamin D-3 in cultured human keratinocytes [J].
Solvsten, H ;
Svendsen, ML ;
Fogh, K ;
Kragballe, K .
ARCHIVES OF DERMATOLOGICAL RESEARCH, 1997, 289 (06) :367-372