Increased α1D adrenergic receptor activity and protein expression in the urinary bladder of aged rats

被引：11

作者：

Dmitrieva, Natalia ^{[1
]}

Zhang, Guohua ^{[1
]}

Nagabukuro, Hiroshi ^{[1
]}

机构：

[1] Florida State Univ, Dept Psychol, Program Neurosci, Tallahassee, FL 32306 USA

来源：

WORLD JOURNAL OF UROLOGY | 2008年 / 26卷 / 06期

关键词：

Ovarian hormone depletion; Urothelium; Suburothelium; Sympathetic nerve; Noradrenalin;

D O I：

10.1007/s00345-008-0292-x

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

A possibility that aging affects (a) expression of the alpha 1D-adrenergic receptor (AR1D), (b) AR1D-mediated contractions and (c) sympathetic innervation in the urinary bladder in rats was studied. Contraction produced by phenylephrine and inhibition of these contractions by a non-selective alpha 1-adrenergic antagonist prazosin and a selective AR1D antagonist BMY7378 were compared between 6- and 24-month-old Fisher rats. Expressions of VMAT and AR1D in the bladder were assessed by immunofluorescence and Western blot. Phenylephrine-induced contractions were larger and inhibition of these contractions by BMY7378 was significantly greater in 24-month-old rats. Aging increased expression of AR1D in the bladder. Density of VMAT-immunoreactive neurites was decreased in smooth muscle but elevated in the suburothelial region of 24-month-old rats. The results suggest that influence of adrenergic activity on bladder contractility increases with aging is due to overexpression of the AR1D. Influence of adrenergic activity on the urothelial function may also be enhanced with aging.

引用

页码：649 / 655

页数：7

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