Genetic polymorphism of Algerian Leishmania infantum strains revealed by multilocus microsatellite analysis

被引：40

作者：

Seridi, Nabila ^{[2
,4
]}

Amro, Ahmad ^{[1
]}

Kuhls, Katrin ^{[1
]}

Belkaid, Miloud ^{[4
]}

Zidane, Charef ^{[3
]}

Al-Jawabreh, Amer ^{[1
]}

Schoenian, Gabriele ^{[1
]}

机构：

[1] Charite, Inst Microbiol & Hyg, D-10117 Berlin, Germany

[2] USTHB, Fac Biol Sci, Genet Lab, Algiers, Algeria

[3] Hosp Mustapha Bacha, Serv Cytol, Ctr Pierre & Marie Curie, Algiers, Algeria

[4] Inst Pasteur, Serv Parasitol, Algiers, Algeria

来源：

MICROBES AND INFECTION | 2008年 / 10卷 / 12-13期

关键词：

Leishmania infantum; Visceral leishmaniasis; Cutaneous leishmaniasis; Microsatellite typing; Algeria; polymorphism;

D O I：

10.1016/j.micinf.2008.07.031

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The present study applies multilocus microsatellite typing (MLMT) for studying the polymorphism among 55 strains of Leishmania infantum from Algeria. These strains from different Algerian foci representing different zymodemes, hosts and clinical forms were analysed using 14 microsatellite markers. All 55 strains had individual MLMT profiles and no relationship was observed between them and different host or geographical origins. Three populations of Algerian L. infantum were identified by a Bayesian clustering approach implemented in STRUCTURE software and supported by genetic distance analysis. Two populations, A and B, consisted mainly of strains belonging to zymodeme MON-1, and the third population, C, mainly of MON-24 strains isolated from cutaneous leishmaniasis cases. Interestingly, a small group of strains appeared as a mixture of different populations and might be putative hybrids. Genetic migration was noticed among the two MON-1 populations, A and B, as well as between populations A and C. Due to its high discriminatory power MLMT could be also successfully applied for differentiating relapses or re-infection for patients suffering from multiple episodes of visceral leishmaniasis. (C) 2008 Elsevier Masson SAS. All fights reserved.

引用

页码：1309 / 1315

页数：7

共 30 条

[11]

IZRI MA, 1990, ANN PARASIT HUM COMP, V65, P151

[12] PHLEBOTOMUS (LARROUSSIUS)-PERFILIEWI NATURALLY INFECTED WITH DERMATROPIC LEISHMANIA-INFANTUM AT TENES, ALGERIA [J].

IZRI, MA ;

BELAZZOUG, S .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1993, 87 (04) :399-399

[13] Differentiation and Gene Flow among European Populations of Leishmania infantum MON-1 [J].

Kuhls, Katrin ;

Chicharro, Carmen ;

Canavate, Carmen ;

Cortes, Sofia ;

Campino, Lenea ;

Haralambous, Christos ;

Soteriadou, Ketty ;

Pratlong, Francine ;

Dedet, Jean-Pierre ;

Mauricio, Isabel ;

Miles, Michael ;

Schaar, Matthias ;

Ochsenreither, Sebastian ;

Radtke, Oliver A. ;

Schoenian, Gabriele .

PLOS NEGLECTED TROPICAL DISEASES, 2008, 2 (07)

[14] Multilocus microsatellite typing (MLMT) reveals genetically isolated populations between and within the main endemic regions of visceral leishmaniasis [J].

Kuhls, Katrin ;

Keilonat, Lyvia ;

Ochsenreither, Sebastian ;

Schaar, Matthias ;

Schweynoch, Carola ;

Presber, Wolfgang ;

Schoenian, Gabriele .

MICROBES AND INFECTION, 2007, 9 (03) :334-343

[15] MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment [J].

Kumar, S ;

Tamura, K ;

Nei, M .

BRIEFINGS IN BIOINFORMATICS, 2004, 5 (02) :150-163

[16]

Lewis P. O., 2001, GENETIC DATA ANAL CO

[17] Towards multilocus sequence typing of the Leishmania donovani complex:: Resolving genotypes and haplotypes for five polymorphic metabolic enzymes (ASAT, GPI, NH1, NH2, PGD) [J].

Mauricio, Isabel L. ;

Yeo, Matthew ;

Baghaei, Mehdi ;

Doto, Daniela ;

Pratlong, Francine ;

Zemanova, Eva ;

Dedet, Jean-Pierre ;

Lukes, Julius ;

Miles, Michael A. .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2006, 36 (07) :757-769

[18] Multiple hybrid genotypes of Leishmania (Viannia) in a focus of mucocutaneous leishmaniasis [J].

Nolder, Debbie ;

Roncal, Norma ;

Davies, Clive R. ;

Llanos-Cuentas, Alejandro ;

Miles, Michael A. .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2007, 76 (03) :573-578

[19] Multilocus microsatellite typing as a new tool for discrimination of Leishmania infantum MON-1 strains [J].

Ochsenreither, S ;

Kuhls, K ;

Schaar, M ;

Presber, W ;

Schönian, G .

JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (02) :495-503

[20] Sudan: the possible original focus of visceral leishmaniasis [J].

Pratlong, F ;

Dereure, J ;

Bucheton, B ;

El-Safi, S ;

Dessein, A ;

Lanotte, G ;

Dedet, JP .

PARASITOLOGY, 2001, 122 :599-605

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