PATTERNS OF mRNA AND PROTEIN EXPRESSION FOR 12 GABAA RECEPTOR SUBUNITS IN THE MOUSE BRAIN

被引：189

作者：

Hoertnagl, H. ^{[1
]}

Tasan, R. O. ^{[1
]}

Wieselthaler, A. ^{[1
]}

Kirchmair, E. ^{[1
]}

Sieghart, W. ^{[2
]}

Sperk, G. ^{[1
]}

机构：

[1] Med Univ Innsbruck, Dept Pharmacol, A-6020 Innsbruck, Austria

[2] Med Univ Vienna, Ctr Brain Res, Dept Biochem & Mol Biol, A-1090 Vienna, Austria

来源：

NEUROSCIENCE | 2013年 / 236卷

基金：

奥地利科学基金会;

关键词：

GABA(A) receptor subunits; mouse brain; in situ hybridization; immunohistochemistry; DELTA-SUBUNIT; RAT-BRAIN; IMMUNOCYTOCHEMICAL DISTRIBUTION; ALTERED EXPRESSION; TONIC INHIBITION; OLFACTORY-BULB; HIPPOCAMPUS; SUSCEPTIBILITY; HETEROGENEITY; SUBTYPES;

D O I：

10.1016/j.neuroscience.2013.01.008

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The GABA(A) receptor is the main inhibitory receptor in the brain and its subunits originate from different genes or gene families (alpha 1-alpha 6, beta 1-beta 3, gamma 1-gamma 3, delta, epsilon, theta, pi, or rho 1-3). In the mouse brain the anatomical distribution of GABA(A) receptor subunit mRNAs so far investigated is restricted to subunits forming benzodiazepine-sensitive receptor complexes (alpha 1-alpha 3, alpha 5, beta 2, beta 3 and gamma 2) in the fore-brain and midbrain as assessed by in situ hybridization (ISH). In the present study the anatomical distribution of the GABA(A) receptor subunits alpha 1-alpha 6, beta 1-beta 3, gamma 1-gamma 2 and delta was analyzed in the mouse brain (excluding brain stem) by ISH and immunohistochemistry (IHC). In several brain areas such as hippocampus, cerebellum, bulbus olfactorius and habenula we observed that mRNA levels did not reflect protein levels, indicating that the protein is located far distantly from the cell body. We also compared the distribution of these 12 subunit mRNAs and proteins with that reported in the rat brain. Although in general there is a considerable correspondence in the distribution between mouse and rat brains, several species-specific differences were observed. (C) 2013 IBRO. Published by Elsevier Ltd. All rights reserved.

引用

页码：345 / 372

页数：28

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