Properties of competitively adsorbed BSA and fibrinogen from their mixture on mixed and hybrid surfaces

被引：31

作者：

Pandey, Lalit M. ^{[1
]}

Pattanayek, Sudip K. ^{[1
]}

机构：

[1] IIT Delhi, Dept Chem Engn, New Delhi 110016, India

来源：

APPLIED SURFACE SCIENCE | 2013年 / 264卷

关键词：

Competitive adsorption; Adsorption of proteins; Chemical potential; Vroman effect; QCM; Hybrid SAM; QUARTZ-CRYSTAL MICROBALANCE; ATOMIC-FORCE MICROSCOPY; HUMAN SERUM-ALBUMIN; PROTEIN ADSORPTION; HYDROPHOBIC SURFACES; PLATELET-ADHESION; PLASMON RESONANCE; POLYMER SURFACES; BLOOD-PLASMA; FIBRONECTIN;

D O I：

10.1016/j.apsusc.2012.10.150

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We have studied the adsorption of BSA and fibrinogen from their mixture onto surfaces with mixed self-assembled monolayer (SAM) of amine and octyl (ratio 1: 1) and hybrid SAM. The properties of adsorbed proteins obtained from individual protein solution differ considerably from the properties of the adsorbed proteins obtained from mixture of proteins at same total concentration. The adsorbed amount of proteins is lesser and the adsorbed protein is more elastic if it is adsorbing from mixture of proteins. It is found that with increasing total protein concentration, adsorbed amount increases and elasticity of the adsorbed proteins decreases. The apparent displacements of BSA with Fb are observed on the graphs of change in frequency with time, which are obtained from quartz crystal microbalance. (C) 2012 Elsevier B. V. All rights reserved.

引用

页码：832 / 837

页数：6

共 26 条

[1] Conformational Transitions of Adsorbed Proteins on Surfaces of Varying Polarity [J].