Spectral unmixing of flavin autofluorescence components in cardiac myocytes

被引：64
作者：
Chorvat, D
Kirchnerova, J
Cagalinec, M
Smolka, J
Mateasik, A
Chorvatova, A
机构：
[1] Ctr Int Laser, Dept Biophoton, Bratislava 81219, Slovakia
[2] Comenius Univ, Fac Math Phys & Informat, Bratislava 84248, Slovakia
[3] Univ Montreal, Dept Pediat, Montreal, PQ H3T 1C5, Canada
[4] Res Ctr St Justine Hosp, Montreal, PQ H3T 1C5, Canada
来源：
BIOPHYSICAL JOURNAL | 2005年 / 89卷 / 06期
基金：
加拿大健康研究院;
关键词：
D O I：
10.1529/biophysj.105.073866
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
We applied linear unmixing approach to reveal individual components of intrinsic flavin fluorescence signal recorded in living cardiac cells by spectrally resolved confocal microscopy. Responses of whole-cell autofluorescence to modulators of cell metabolism and respiration were used as a tool of separation of its components; their spectral profiles, estimated by principal component analysis, correspond to free FAD and FAD bound to different enzymes of electron transport chain.
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页码：L55 / L57
页数：3
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