Ammonia-oxidizing archaea and nitrite-oxidizing nitrospiras in the biofilter of a shrimp recirculating aquaculture system

被引：89

作者：

Brown, Monisha N. ^{[1
]}

Briones, Aurelio ^{[2
]}

Diana, James ^{[3
]}

Raskin, Lutgarde ^{[1
]}

机构：

[1] Univ Michigan, Dept Civil & Environm Engn, Ann Arbor, MI 48109 USA

[2] Univ Idaho, Dept Plant Soil & Entomol Sci, Moscow, ID 83843 USA

[3] Univ Michigan, Sch Nat Resources & Environm, Ann Arbor, MI 48109 USA

来源：

FEMS MICROBIOLOGY ECOLOGY | 2013年 / 83卷 / 01期

基金：

美国国家科学基金会;

关键词：

ammonia oxidation; nitrite oxidation; Nitrospira; Nitrosopumilus maritimus; shrimp aquaculture; recirculating aquaculture system; 16S RIBOSOMAL-RNA; WASTE-WATER TREATMENT; VANNAMEI BOONE JUVENILES; NITRIFICATION PERFORMANCE; ACUTE TOXICITY; SP-NOV; BACTERIA; OXIDATION; DIVERSITY; COMMUNITY;

D O I：

10.1111/j.1574-6941.2012.01448.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This study analysed the nitrifier community in the biofilter of a zero discharge, recirculating aquaculture system (RAS) for the production of marine shrimp in a low density (low ammonium production) system. The ammonia-oxidizing populations were examined by targeting 16S rRNA and amoA genes of ammonia-oxidizing bacteria (AOB) and archaea (AOA). The nitrite-oxidizing bacteria (NOB) were investigated by targeting the 16S rRNA gene. Archaeal amoA genes were more abundant in all compartments of the RAS than bacterial amoA genes. Analysis of bacterial and archaeal amoA gene sequences revealed that most ammonia oxidizers were related to Nitrosomonas marina and Nitrosopumilus maritimus. The NOB detected were related to Nitrospira marina and Nitrospira moscoviensis, and Nitrospira marina-type NOB were more abundant than N. moscoviensis-type NOB. Water quality and biofilm attachment media played a role in the competitiveness of AOA over AOB and Nitrospira marina-over N. moscoviensis-type NOB.

引用

页码：17 / 25

页数：9

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