Acetylcholinesterase inhibition, antioxidant activity and toxicity of Peumus boldus water extracts on HeLa and Caco-2 cell lines

被引：28
作者：
Fale, P. L. [1 ,2 ]
Amaral, F. [3 ]
Amorim Madeira, P. J. [1 ]
Silva, M. Sousa [1 ]
Florencio, M. H. [1 ,3 ]
Frazao, F. N. [1 ,3 ,4 ]
Serralheiro, M. L. M. [1 ,3 ]
机构：
[1] Univ Lisbon, Fac Ciencias, Ctr Quim & Bioquim, P-1749016 Lisbon, Portugal
[2] Univ Lisbon, Fac Ciencias, Ctr Biotecnol Vegetal IBB, P-1749016 Lisbon, Portugal
[3] Univ Lisbon, Fac Ciencias, Dept Quim & Bioquim, P-1749016 Lisbon, Portugal
[4] Univ Lusofona Humanidades & Tecnol, Dept Ciencias Saude, Lisbon, Portugal
来源：
FOOD AND CHEMICAL TOXICOLOGY | 2012年 / 50卷 / 08期
关键词：
Peumus boldus; Aqueous extracts; Acetylcholinesterase inhibition; Antioxidant; HeLa toxicity; In vitro digestion; ANTIACETYLCHOLINESTERASE; FLAVONOIDS; BOLDINE;
D O I：
10.1016/j.fct.2012.04.049
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
This work aimed to study the inhibition on acetylcholinesterase activity (AChE), the antioxidant activity and the toxicity towards Caco-2 and HeLa cells of aqueous extracts of Peumus Boldus. An IC50 value of 0.93 mg/mL, for AChE inhibition, and EC50 of 18.7 mu g/mL, for the antioxidant activity, was determined. This activity can be attributed to glycosylated flavonoid derivatives detected, which were the main compounds, although boldine and other aporphine derivatives were also present. No changes in the chemical composition or the biochemical activities were found after gastrointestinal digestion. Toxicity of P. boldus decoction gave an IC50 value 0.66 mg/mL for HeLa cells, which caused significant changes in the cell proteome profile. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：2656 / 2662
页数：7
相关论文
共 32 条
[1]   Antitumor activity of some natural flavonoids and synthetic derivatives on various human and murine cancer cell lines [J].
Cárdenas, M ;
Marder, M ;
Blank, VC ;
Roguin, LP .
BIOORGANIC & MEDICINAL CHEMISTRY, 2006, 14 (09) :2966-2971
[2]   Cytotoxic effects of propiconazole and its metabolites in mouse and human hepatoma cells and primary mouse hepatocytes [J].
Chen, Pei-Jen ;
Moore, Tanya ;
Nesnow, Stephen .
TOXICOLOGY IN VITRO, 2008, 22 (06) :1476-1483
[3]   Availability of polyphenols in fruit beverages subjected to in vitro gastrointestinal digestion and their effects on proliferation, cell-cycle and apoptosis in human colon cancer Caco-2 cells [J].
Cilla, Antonio ;
Gonzalez-Sarrias, Antonio ;
Tomas-Barberan, Francisco A. ;
Espin, Juan Carlos ;
Barbera, Reyes .
FOOD CHEMISTRY, 2009, 114 (03) :813-820
[4]   Antiproliferative Effect of Flavonoids and Sesquiterpenoids from Achillea millefolium s.l. on Cultured Human Tumour Cell Lines [J].
Csupor-Loeffler, Boglarka ;
Hajdu, Zsuzsanna ;
Zupko, Istvan ;
Rethy, Borbala ;
Falkay, George ;
Forgo, Peter ;
Hohmann, Judit .
PHYTOTHERAPY RESEARCH, 2009, 23 (05) :672-676
[5]  
de Almeida ER, 2000, PHYTOTHER RES, V14, P99, DOI 10.1002/(SICI)1099-1573(200003)14:2<99::AID-PTR600>3.0.CO
[6]  
2-4
[7]   Rosmarinic acid, scutellarein 4′-methyl ether 7-O-glucuronide and (16S)-coleon E are the main compounds responsible for the antiacetylcholinesterase and antioxidant activity in herbal tea of Plectranthus barbatus ("falso boldo") [J].
Fale, Pedro L. ;
Borges, Carlos ;
Amorim Madeira, Paulo J. ;
Ascensao, Lia ;
Araujo, Maria Eduarda M. ;
Florencio, Maria Helena ;
Serralheiro, Maria Luisa M. .
FOOD CHEMISTRY, 2009, 114 (03) :798-805
[8]   Protein profile of the HeLa cell line [J].
Fountoulakis, M ;
Tsangaris, G ;
Oh, JE ;
Maris, A ;
Lubec, G .
JOURNAL OF CHROMATOGRAPHY A, 2004, 1038 (1-2) :247-265
[9]   Classical cadherin adhesion molecules: coordinating cell adhesion, signaling and the cytoskeleton [J].
Goodwin, M ;
Yap, AS .
JOURNAL OF MOLECULAR HISTOLOGY, 2004, 35 (8-9) :839-844
[10]   Acetylcholinesterase inhibition and antioxidant activity of the water extracts of several Hypericum species [J].
Hernandez, Maria Filipa ;
Fale, Pedro L. V. ;
Araujo, Maria Eduarda M. ;
Serralheiro, Maria Luisa M. .
FOOD CHEMISTRY, 2010, 120 (04) :1076-1082
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