Genetic imprinting of H19 and IGF2 in domestic pigs (Sus scrofa)

被引：22

作者：

Li, Chao ^{[1
]}

Bin, Yanfang ^{[1
]}

Curchoe, Carol ^{[2
]}

Yang, Lan ^{[2
]}

Feng, Dingyuan ^{[1
]}

Jiang, Qingyan ^{[1
]}

O'Neill, Michael ^{[3
]}

Tian, X. Cindy ^{[2
]}

Zhang, Shouquan ^{[1
]}

机构：

[1] S China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China

[2] Univ Connecticut, Dept Anim Sci, Ctr Regenerat Biol, Storrs, CT 06269 USA

[3] Univ Connecticut, Dept Mol & Cellular Biol, Storrs, CT 06269 USA

来源：

ANIMAL BIOTECHNOLOGY | 2008年 / 19卷 / 01期

基金：

中国国家自然科学基金;

关键词：

H19; IGF2; imprinting; porcine;

D O I：

10.1080/10495390701758563

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The genes insulin-like growth factor 2 (IGF2) and H19 express paternally and maternally, respectively, in humans, mice, sheep, and cattle. Additionally, IGF2 has been shown to be regulated by at least four promoters in a tissue- or development-specific manner. In the domestic pigs, the promoter- and tissue-specific imprinting pattern of IGF2 has not been well characterized, nor is the imprinting pattern of H19. In the present study, we identified two polymorphisms in each of IGF2 (exons 2 and 9) and H19 (exons 1 and 5) and determined the imprinting status of these two genes in 13 organs / tissues of week-old pigs. IGF2 P1 transcript is bi-allelically expressed (not imprinted) in all major organs studied, while the majority of IGF2 transcripts are expressed from promoters 2-4 and are imprinted. H19 is exclusively expressed from the maternal allele in all major organs, concurrent with observations in other species.

引用

页码：22 / 27

页数：6

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