Mitochondria-Accessing Ratiometric Fluorescent Probe for Imaging Endogenous Superoxide Anion in Live Cells and Daphnia magna

被引:71
作者
Zhang, Zhen [1 ]
Fan, Jiangli [1 ]
Zhao, Yuhui [1 ]
Kang, Yao [1 ]
Du, Jianjun [1 ]
Peng, Xiaojun [1 ]
机构
[1] Dalian Univ Technol, State Key Lab Fine Chem, 2 Linggong Rd, Dalian 116024, Peoples R China
来源
ACS SENSORS | 2018年 / 3卷 / 03期
基金
美国国家科学基金会;
关键词
superoxide anion; mitochondria-accessing; ratiometric bioimaging; fluorescent probe; fast response; inflammatory Daphnia magna; LIVING CELLS; IN-VIVO; HYDROGEN-PEROXIDE; LIPID-PEROXIDATION; OXIDATIVE STRESS; SINGLET OXYGEN; FREE-RADICALS; GENERATION; TRANSDUCTION; INFLAMMATION;
D O I
10.1021/acssensors.8b00082
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Superoxide anion (O-2(center dot-)), as the precursor of other reactive oxygen species (ROS), is significantly important in the maintenance of redox homeostasis and various cellular signaling pathways. Here we present a ratiometric mitochondria-accessing fluorescent probe (NA-T) based on nucleophilic substitution mechanism for real-time measuring O-2(center dot-). By regulating the intramolecular charge of 1,8-naphthalimide, a ratiometric response model was obtained, which evinced 18 fold enhancement of fluorescence ratio (I-540 (nm)/I-475 (nm)) in the presence of O-2(center dot-) over other ROS with rapid response (132 s), high sensitivity (DL = 0.370 mu M) and selectivity. Confocal fluorescence images demonstrated that the probe could well permeate through plasma membrane for visualizing endogenous O-2(center dot-) changes in mitochondria of living cells and in inflammatory Daphnia magna, indicating NA-T a potential tool for the diagnosis and research of corresponding diseases.
引用
收藏
页码:735 / 741
页数:13
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