Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9-sgRNA Ribonucleoproteins

被引：209
作者：
Cho, Seung Woo [1 ]
Lee, Jihyun [2 ]
Carroll, Dana [4 ]
Kim, Jin-Soo [1 ]
Lee, Junho [2 ,3 ]
机构：
[1] Seoul Natl Univ, Dept Chem, Seoul 151742, South Korea
[2] Seoul Natl Univ, Dept Biol Sci, Seoul 151742, South Korea
[3] Seoul Natl Univ, Dept Biophys & Chem Biol, Seoul 151742, South Korea
[4] Univ Utah, Sch Med, Dept Biochem, Salt Lake City, UT 84112 USA
来源：
GENETICS | 2013年 / 195卷 / 03期
基金：
新加坡国家研究基金会; 美国国家卫生研究院;
关键词：
Caenorhabditis elegans; Cas9-sgRNA ribonucleoprotein; NHEJ; genome editing; HUMAN-CELLS; GENOME; UNC-1;
D O I：
10.1534/genetics.113.155853
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
We present a novel method of targeted gene disruption that involves direct injection of recombinant Cas9 protein complexed with guide RNA into the gonad of the nematode Caenorhabditis elegans. Biallelic mutants were recovered among the F-1 progeny, demonstrating the high efficiency of this method.
引用
收藏
页码：1177 / +
页数：5
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