Targeting enzymes to the right compartment: Metabolic engineering for itaconic acid production by Aspergillus niger

被引:85
作者
Blumhoff, Marzena L. [1 ,2 ]
Steiger, Matthias G. [1 ,3 ]
Mattanovich, Diethard [1 ,3 ]
Sauer, Michael [1 ,3 ]
机构
[1] BOKU VIBT Univ Nat Resources & Life Sci, Dept Biotechnol, A-1190 Vienna, Austria
[2] Univ Appl Sci FH, Sch Bioengn, Vienna, Austria
[3] Austrian Ctr Ind Biotechnol ACIB GmbH, Vienna, Austria
关键词
Mitochondrial targeting; cis-Aconitate decarboxylase; Aconitase; Microbial organic acid production; AcoA; CadA; CITRATE PRODUCTION; EXPRESSION; GENE; PATHWAYS; DECARBOXYLASE; CONSTRUCTION; FERMENTATION; MORPHOLOGY; NIDULANS; PROTEIN;
D O I
10.1016/j.ymben.2013.05.003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Itaconic acid is an unsaturated dicarboxylic acid which has a high potential as a biochemical building block, It can be microbially produced from some Aspergillus species, such as Aspergillus itaconicus and Aspergillus terreus. However, the achieved titers are significantly lower as compared to the citric acid production by A. niger. Heterologous expression of cis-aconitate decarboxylase in A. niger leads to the accumulation of small amounts of itaconic acid. Additional expression of aconitase, the second enzyme metabolically linking citric acid and itaconic acid improves productivity. However, proper organelle targeting of the enzymes appears to be an important point to consider. Here we compare the mitochondrial expression with the cytosolic expression of cis-aconitate decarboxylase or aconitase in A niger. Heterologous expression of both enzymes in the mitochondria doubles the productivity compared to strains which express the enzymes in the cytosol. It is essential to target enzymes to the correct compartment in order to establish a proper flux through a compartmentalized pathway. (C) 2013 Elsevier Inc. All rights reserved.
引用
收藏
页码:26 / 32
页数:7
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