Mitochondrial dysfunction leads to reduced chronological lifespan and increased apoptosis in yeast

被引：60

作者：

Aerts, An M. ^{[1
]}

Zabrocki, Piotr ^{[2
]}

Govaert, Gilmer ^{[1
]}

Mathys, Janick ^{[1
]}

Carmona-Gutierrez, Didac ^{[3
]}

Madeo, Frank ^{[3
]}

Winderickx, Joris ^{[2
]}

Cammue, Bruno P. A. ^{[1
]}

Thevissen, Karin ^{[1
]}

机构：

[1] Katholieke Univ Leuven, CMPG, B-3001 Heverlee, Belgium

[2] Katholieke Univ Leuven, LFB, B-3001 Heverlee, Belgium

[3] Karl Franzens Univ Graz, IMB, A-8010 Graz, Austria

来源：

FEBS LETTERS | 2009年 / 583卷 / 01期

关键词：

Apoptosis; Chronological lifespan; Microarray analysis; Mitochondrial function; Mitochondrial morphology; Respiration; SACCHAROMYCES-CEREVISIAE; GENE-EXPRESSION; INCREASED RESPIRATION; MUTANTS; IDENTIFICATION; INTERMEDIATE; DNA;

D O I：

10.1016/j.febslet.2008.11.028

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We previously isolated a Saccharomyces cerevisiae mutant (HsTnII), which displays 40% reduced chronological lifespan as compared to the wild type (WT). In this study, we found HsTnII cultures to be characterized by fragmented and dysfunctional mitochondria, and by increased initiation of apoptosis during chronological aging as compared to WT. Expression of genes encoding subunits of mitochondrial electron transport chain and ATP synthase is significantly downregulated in HsTnII, and as a consequence, HsTnII is not able to respire ethanol. All these data confirm the importance of functional mitochondria and respiration in determining yeast chronological lifespan and apoptosis. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：113 / 117

页数：5

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