Human placental cells show enhanced production of interleukin (IL)-8 in response to lipopolysaccharide (LPS), IL-1 and tumour necrosis factor (TNF)-α, but not to IL-6

被引:39
作者
Shimoya, K
Moriyama, A
Matsuzaki, N
Ogata, I
Koyama, M
Azuma, C
Saji, F
Murata, Y
机构
[1] Osaka Univ, Fac Med, Dept Obstet & Gynecol, Suita, Osaka 5650871, Japan
[2] Osaka Police Hosp, Dept Obstet & Gynecol, Tennoji Ku, Osaka 5438502, Japan
[3] Ikeda City Hosp, Dept Obstet & Gynecol, Ikeda, Osaka 5630025, Japan
[4] Osaka Med Ctr Canc & Cardiovasc Dis, Dept Gynecol, Higashinari Ku, Osaka 5370025, Japan
关键词
cytokine; IL-8; LPS; placental cell; trophoblast;
D O I
10.1093/molehr/5.9.885
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Interleukin-8 (IL-8) is a chemotactic and activating factor for neutrophils which play important roles in host defence mechanisms. The human placenta constitutively produces IL-8 during pregnancy and enhances its production in chorioamnionitis. The present study was designed to investigate in vitro the regulatory mechanism for IL-8 production in the placentas in normal and inflammatory states. Placental cells produced IL-8 in a dose-dependent fashion when stimulated with lipopolysaccharide (LPS). The purified trophoblasts showed significantly higher IL-8 production than untreated placental cells. The expression of IL-8 gene in the trophoblasts in the third trimester was observed by reverse transcription-polymerase chain reaction (RT-PCR). The placental cells also release IL-8 in a dose-dependent manner, in response to r-(recombinant) IL-1 alpha and tumour necrosis factor (TNF)-alpha, but not rIL-6. Moreover, LPS-activated placental cells spontaneously produced a much larger amount of IL-8 and showed increased responses to rIL-1 alpha and TNF-alpha. It may, therefore, be proposed that placental cells with multiple endocrine functions exert immunological functions by constitutive production of IL-1 and TNF-alpha, which stimulate placental IL-8 release. This cytokine cascade in the placenta may be augmented by LPS in chorioamnionitis, thereby potentiating the fete-maternal defence mechanisms against infection.
引用
收藏
页码:885 / 891
页数:7
相关论文
共 41 条
  • [1] NEUTROPHIL-ACTIVATING PEPTIDE-1 INTERLEUKIN-8, A NOVEL CYTOKINE THAT ACTIVATES NEUTROPHILS
    BAGGIOLINI, M
    WALZ, A
    KUNKEL, SL
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1989, 84 (04) : 1045 - 1049
  • [2] CASSATELLA MA, 1992, J IMMUNOL, V148, P3216
  • [3] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
  • [4] PLATELET-ACTIVATING-FACTOR AS A STIMULUS OF EXOCYTOSIS IN HUMAN-NEUTROPHILS
    DEWALD, B
    BAGGIOLINI, M
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 888 (01) : 42 - 48
  • [5] TUMOR-NECROSIS-FACTOR (CACHECTIN) IS AN ENDOGENOUS PYROGEN AND INDUCES PRODUCTION OF INTERLEUKIN-1
    DINARELLO, CA
    CANNON, JG
    WOLFF, SM
    BERNHEIM, HA
    BEUTLER, B
    CERAMI, A
    FIGARI, IS
    PALLADINO, MA
    OCONNOR, JV
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1986, 163 (06) : 1433 - 1450
  • [6] DJEU JY, 1990, J IMMUNOL, V144, P2205
  • [7] ISOLATION OF PURE VILLOUS CYTOTROPHOBLAST FROM TERM HUMAN-PLACENTA USING IMMUNOMAGNETIC MICROSPHERES
    DOUGLAS, GC
    KING, BF
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 119 (02) : 259 - 268
  • [8] EMBER JA, 1992, J IMMUNOL, V148, P3165
  • [9] GOODMAN RB, 1992, J IMMUNOL, V148, P457
  • [10] HUNT JS, 1989, J IMMUNOL, V143, P1606