Efficient production of 1,3-propanediol from glycerol upon constitutive expression of the 1,3-propanediol oxidoreductase gene in engineered Klebsiella pneumoniae with elimination of by-product formation

被引：14

作者：

Oh, Baek-Rock ^{[1
,2
]}

Seo, Jeong-Woo ^{[1
]}

Heo, Sun-Yeon ^{[1
]}

Luo, Lian Hua ^{[1
,3
]}

Hong, Won-Kyung ^{[1
]}

Park, Don-Hee ^{[1
,2
]}

Kim, Chul-Ho ^{[1
]}

机构：

[1] KRIBB, Jeonbuk Branch Inst, Microbe Based Fus Technol Res Ctr, Jeongeup 580185, Jeonbuk, South Korea

[2] Chonnam Natl Univ, Grad Sch Bioenergy & Biomat, Interdisciplinary Program, Kwangju 500757, South Korea

[3] Chonbuk Natl Univ, Inst Mol Biol & Genet, Res Ctr Bioact Mat, Jeonju 561756, Chonbuk, South Korea

来源：

BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2013年 / 36卷 / 06期

关键词：

1,3-Propanediol; Glycerol; Constitutive expression; Klebsiella pneumoniae; By-product formation; MICROBIAL-PRODUCTION;

D O I：

10.1007/s00449-013-0901-y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In the present study, we developed an efficient method of 1,3-propanediol (1,3-PD) production from glycerol by genetic engineering of Klebsiella pneumoniae AK mutant strains. The proposed approach eliminated by-product formation and IPTG induction resulted in maximal production of 1,3-PD. A series of recombinant strains was designed to constitutively express the dhaB and/or dhaT genes, using the bacteriophage T5 P-DE20 promoter and the rho-independent transcription termination signal of the Rahnella aquatilis levansucrase gene. Among these strains, AK/pConT expressing dhaT alone gave the highest yield of 1,3-PD. Fed-batch fermentation resulted in efficient production of 1,3-PD from either pure or crude glycerol, without by-product formation.

引用

页码：757 / 763

页数：7

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