Ethanol Extract of Cissus quadrangularis Enhances Osteoblast Differentiation and Mineralization of Murine Pre-Osteoblastic MC3T3-E1 Cells

被引:21
作者
Tasadduq, Raazia [1 ,2 ]
Gordon, Jonathan [2 ]
Al-Ghanim, Khalid A. [3 ]
Lian, Jane B. [2 ]
Van Wijnen, Andre J. [4 ]
Stein, Janet L. [2 ]
Stein, Gary S. [2 ]
Shakoori, Abdul Rauf [1 ,3 ]
机构
[1] Univ Punjab, Sch Biol Sci, Quaid I Azam Campus, Lahore 54590, Pakistan
[2] Univ Vermont, Coll Med, Dept Biochem, Burlington, VT USA
[3] King Saud Univ, Coll Sci, Dept Zool, Riyadh, Saudi Arabia
[4] Mayo Clin, Dept Orthoped Surg & Biochem & Mol Biol, Rochester, MN USA
关键词
ALKALINE-PHOSPHATASE ACTIVITY; PETROLEUM-ETHER EXTRACT; IN-VITRO; OSTEOPOROSIS; PROLIFERATION; ACTIVATION; KAEMPFEROL; QUERCETIN; LINN; BONE;
D O I
10.1002/jcp.25449
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Traditional medicinal literature and previous studies have reported the possible role of Cissus quadrangularis (CQ) as an anti-osteoporotic agent. This study examines the effectiveness of CQ in promoting osteoblast differentiation of the murine pre-osteoblast cell line, MC3T3-E1. Ethanolic extract of CQ (CQ-E) was found to affect growth kinetics of MC3T3-E1 cells in a dosage-dependent manner. High concentrations of CQ-E (more than 10g/ml) have particularly adverse effects, while lower concentrations of 0.1 and 1 mu g/ml were non-toxic and did not affect cell viability. Notably, cell proliferation was significantly increased at the lower concentrations of CQ-E. CQ-E treatment also augmented osteoblast differentiation, as reflected by a substantial increase in expression of the early osteoblast marker ALP activity, and at later stage, by mineralization of extracellular matrix compared to the control group. These findings suggest dose-dependent effect of CQ-E with lower concentrations exhibiting anabolic and osteogenic properties. J. Cell. Physiol. 232: 540-547, 2017. (c) 2016 Wiley Periodicals, Inc.
引用
收藏
页码:540 / 547
页数:8
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