Single Cell Gel Electrophoresis (Comet) assay with plants: Research on DNA repair and ecogenotoxicity testing

被引：47

作者：

Ventura, Lorenzo ^{[1
]}

Giovannini, Annalisa ^{[2
]}

Savio, Monica ^{[3
]}

Dona, Mattia ^{[4
]}

Macovei, Anca ^{[4
]}

Buttafava, Armando ^{[1
]}

Carbonera, Daniela ^{[4
]}

Balestrazzi, Alma ^{[4
]}

机构：

[1] Dipartimento Chim, I-27100 Pavia, Italy

[2] Consiglio Ric & Sperimentaz Agr, Unita Ric Floricoltura & Specie Ornamentali CRA F, I-18038 San Remo, IM, Italy

[3] Dipartimento Med Sperimentale, Sez Patol Gen C Golgi, I-27100 Pavia, Italy

[4] Dipartimento Biol & Biotecnol L Spallanzani, Lab Genet & Microbiol A Buzzati Traverso, I-27100 Pavia, Italy

来源：

CHEMOSPHERE | 2013年 / 92卷 / 01期

关键词：

Biomonitoring; DNA repair; Environmental pollution; Genotoxic stress; Ionizing radiation; SCGE; POLYCYCLIC AROMATIC-HYDROCARBONS; IN-SITU HYBRIDIZATION; DOUBLE-STRAND BREAKS; HOMOLOGOUS RECOMBINATION; OXIDATIVE DAMAGE; MALEIC HYDRAZIDE; CYCLE; FISH; GENOTOXICITY; VITRO;

D O I：

10.1016/j.chemosphere.2013.03.006

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Single Cell Gel Electrophoresis is currently used to investigate the cell response to genotoxic agents as well as to several biotic and abiotic stresses that lead to oxidative DNA damage. Different versions of Single Cell Gel Electrophoresis have been developed in order to expand the range of DNA lesions that can be detected and guidelines for their use in genetic toxicology have been provided. Applications of Single Cell Gel Electrophoresis in plants are still limited, compared to animal systems. This technique is now emerging as a useful tool in assessing the potential of higher plants as stable sensors in ecosystems and source of information on the genotoxic impact of dangerous pollutants. Another interesting application of Single Cell Gel Electrophoresis deals with Mutation Breeding or the combined use of irradiation and in vitro culture technique to enhance genetic variability in elite plant genotypes. SCGE, in combination with in situ detection of Reactive Oxygen Species (ROS) induced by gamma-rays and expression analysis of both DNA repair and antioxidant genes, can be used to gather information on the radiosensitivity level of the target plant genotypes. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：1 / 9

页数：9

共 97 条

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