共 33 条
Comparative proteome analysis of Tumor necrosis factor α-stimulated human Vascular Smooth Muscle Cells in response to melittin
被引:11
作者:
Cho, Hyun-Ji
[1
,2
]
Kang, Jeong-Han
[3
]
Park, Kwan-Kyu
[1
,2
]
Choe, Jung-Yoon
[1
,2
]
Park, Yoon-Yub
[1
,2
]
Moon, Yong-Suk
[1
,2
]
Chung, Il-Kyung
[4
]
Chang, Hyeun-Wook
[5
]
Kim, Cheorl-Ho
[6
]
Choi, Yung Hyun
[7
]
Kim, Wun-Jae
[8
]
Moon, Sung-Kwon
[9
]
Chang, Young-Chae
[1
,2
]
机构:
[1] Catholic Univ Daegu, Sch Med, Res Inst Biomed Engn, Taegu 705718, South Korea
[2] Catholic Univ Daegu, Sch Med, Dept Med, Taegu 705718, South Korea
[3] NCI, Cell Biol Lab, Natl Inst Hlth, Bethesda, MD 20892 USA
[4] Catholic Univ Daegu, Dept Biotechnol, Gyongsan 712702, South Korea
[5] Yeungnam Univ, Coll Pharm, Gyongsan 712749, South Korea
[6] Sungkyunkwan Univ, Dept Biol Sci, Sungkyunkwan 440746, Kyunggi Do, South Korea
[7] Dong Eui Univ, Coll Oriental Med, Dept Biochem, Pusan 614052, South Korea
[8] Chungbuk Natl Univ, Personalized Tumor Engn Res Ctr, Dept Urol, Cheongju 361763, Chungbuk, South Korea
[9] Chung Ang Univ, Dept Food Sci & Technol, Ansung 456756, South Korea
来源:
基金:
新加坡国家研究基金会;
关键词:
INDUCED MIGRATION;
IN-VITRO;
PROHIBITIN;
PEPTIDE;
NEUTROPHILS;
ARTHRITIS;
STRESS;
D O I:
10.1186/1477-5956-11-20
中图分类号:
Q5 [生物化学];
学科分类号:
071010 ;
081704 ;
摘要:
Background: Bee venom has been used to relieve pain and to treat inflammatory diseases, including rheumatoid arthritis, in humans. To better understand the mechanisms of the anti-inflammatory and anti-atherosclerosis effect of bee venom, gel electrophoresis and mass spectrometry were used to identify proteins whose expression was altered in human Vascular Smooth Muscle Cells (hVSMCs) stimulated by tumor necrosis factor alpha after 12 h in the presence of melittin. Results: To obtain valuable insights into the anti-inflammatory and anti-atherosclerosis mechanisms of melittin, two-dimensional (2-D) gel electrophoresis and MALDI-TOF/TOF were used. The proteome study, we showed 33 significant proteins that were differentially expressed in the cells treated with tumor necrosis factor alpha and melittin. Thirteen proteins were significantly increased in the cells treated with tumor necrosis factor alpha, and those proteins were reduced in the cells treated with melittin. Five of the proteins that showed increased expression in the cells treated with tumor necrosis factor alpha are involved in cell migration, including calreticulin, an essential factor of development that plays a role in transcription regulation. The proteins involved in cell migration were reduced in the melittin treated cells. The observed changes in the expression of GRP75, prohibitin, and a select group of other proteins were validated with reverse transcribed-PCR. It was confirmed that the observed change in the protein levels reflected a change in the genes level. In addition, the phosphorylation of EGFR and ERK was validated by analyzing the protein pathway. Conclusion: Taken together, these data established that the expression of some proteins was significantly changed by melittin treatment in tumor necrosis factor alpha stimulated the cells and provided insights into the mechanism of the melittin function for its potential use as an anti-inflammatory agent.
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页数:9
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