Bench-to-bedside review: Rapid molecular diagnostics for bloodstream infection - a new frontier?

被引：95

作者：

Afshari, Arash ^{[1
,2
]}

Schrenzel, Jacques ^{[3
]}

Ieven, Margareta ^{[4
]}

Harbarth, Stephan ^{[1
]}

机构：

[1] Geneva Univ Hosp & Med Sch, Infect Control Program, CH-1211 Geneva 14, Switzerland

[2] Rigshosp, Copenhagen Univ Hosp, Juliane Marie Ctr, Dept Anaesthesia, DK-2100 Copenhagen, Denmark

[3] Geneva Univ Hosp & Med Sch, Clin Microbiol Lab, CH-1211 Geneva 14, Switzerland

[4] Univ Antwerp, Vaccine & Infect Dis Inst, Lab Med Microbiol, Univ Antwerp Hosp, B-2650 Antwerp, Belgium

来源：

CRITICAL CARE | 2012年 / 16卷 / 03期

关键词：

REAL-TIME PCR; POLYMERASE-CHAIN-REACTION; IONIZATION-MASS-SPECTROMETRY; IN-SITU HYBRIDIZATION; COAGULASE-NEGATIVE STAPHYLOCOCCI; LIGHTCYCLER SEPTIFAST TEST; GENEOHM STAPHSR ASSAY; GRAM-POSITIVE COCCI; MULTIPLEX PCR; METHICILLIN-RESISTANT;

D O I：

10.1186/cc11202

中图分类号：

R4 [临床医学];

学科分类号：

1002 ; 100602 ;

摘要：

Among critically ill patients, the diagnosis of bloodstream infection poses a major challenge. Current standard bacterial identification based on blood culture platforms is intrinsically time-consuming and slow. The continuous evolvement of molecular techniques has the potential of providing a faster, more sensitive and direct identification of causative pathogens without prior need for cultivation. This may ultimately impact clinical decision-making and antimicrobial treatment. This review summarises the currently available technologies, their strengths and limitations and the obstacles that have to be overcome in order to develop a satisfactory bedside point-of-care diagnostic tool for detection of bloodstream infection.

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页数：12

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