Dibenzazepin hydrochloride as a new spectrophotometric reagent for determination of hydrogen peroxide in plant extracts

被引：14

作者：

Nagaraja, P. ^{[2
]}

Prakash, J. S. ^{[3
]}

Asha, S. C. ^{[3
]}

Bhaskara, B. L. ^{[1
]}

Kumar, S. Anil ^{[1
]}

机构：

[1] Amrita Vishwa Vidyapeetham, Amrita Sch Engn, Dept Chem, Bangalore 560035, Karnataka, India

[2] Univ Mysore, Dept Studies Chem, Mysore 570006, Karnataka, India

[3] Sri Bhagawan Mahaveer Jain Coll Engn, Dept Chem, Jakkasandra 562112, Karnataka, India

来源：

ENVIRONMENTAL MONITORING AND ASSESSMENT | 2012年 / 184卷 / 10期

关键词：

Hydrogen peroxide; Dibenzazepin hydrochloride; Ampyrone; Visible spectrophotometry; Oxidative coupling; Plant extracts; OXIDATIVE STRESS; METABOLISM; SENESCENCE; LIGHT; WATER;

D O I：

10.1007/s10661-011-2395-x

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A rapid, simple, accurate, and sensitive visible spectrophotometric method for the determination of trace amounts of hydrogen peroxide in acidic buffer medium is reported. The proposed method is based on the oxidative coupling of Ampyrone with dibenzazepin hydrochloride by hydrogen peroxide in the buffer medium of pH 4.0 which is catalyzed by ferrous iron. The blue-colored product formed with maximum absorption at 620 nm was found to be stable for 2 h. Beer's law is obeyed for hydrogen peroxide concentration in the range of 0.03-0.42 mu g ml(-1). The optimum reaction conditions and other important optical parameters are reported. The molar absorptive and Sandell's sensitivity are found to be 5.89 x 10(4) mol(-1) cm(-1) and 0.57 g/cm(2), respectively. The interference due to diverse ions and complexing agents was studied. The method is successfully applied to the determination of hydrogen peroxide in green plants satisfactorily.

引用

页码：5983 / 5988

页数：6

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