Identification of potato cyst nematode species Globodera rostochiensis and G-pallida by PCR using specific primer combinations

被引：41
作者：
Fullaondo, A
Barrena, E
Viribay, M
Barrena, I
Salazar, A
Ritter, E
机构：
[1] Ctr Invest & Mejora Agr Granja Modelo, Vitoria 01080, Spain
[2] Hosp Ramon y Cajal, Unidad Genet Mol, E-28034 Madrid, Spain
来源：
NEMATOLOGY | 1999年 / 1卷
关键词：
cyst nematodes; Globodera; PCR; RAPD; species diagnosis;
D O I：
10.1163/156854199508126
中图分类号：
Q95 [动物学];
学科分类号：
071002 ;
摘要：
RAPD fragments specific for potato cyst nematode (PCN) species Globodera rostochiensis and G. pallida were identified. These were used to derive two primer combinations for PCR which amplify only one specific band in each species. The primer combinations were evaluated on 39 samples of PCN populations containing all currently described pathotypes as well as some South American PCN populations. This method for species identification is highly sensitive and also allows the detection of species mixtures in a sample. Furthermore, the generation of unique amplification products facilitates the application of automatic detection methods without electrophoretic separation and is therefore particularly useful for routine diagnoses.
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页码：157 / 163
页数：7
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