Microbial corrosion monitoring by an amperometric microbial biosensor developed using whole cell of Pseudomonas sp.

被引：19

作者：

Dubey, RS ^{[1
]}

Upadhyay, SN ^{[1
]}

机构：

[1] Banaras Hindu Univ, Ctr Adv Study, Dept Chem Engn & Technol, Varanasi 221005, Uttar Pradesh, India

来源：

BIOSENSORS & BIOELECTRONICS | 2001年 / 16卷 / 9-12期

关键词：

immobilization; microbial biosensor; microbial corrosion; Pseudomonas sp; Thiobacillus sp;

D O I：

10.1016/S0956-5663(01)00203-2

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A microbial biosensor was developed for monitoring microbiologically influenced corrosion (MIC) of metallic materials in industrial systems. The Pseudomonas sp. isolated from corroded metal surface was immobilized on acetylcellulose membrane and its respiratory activity was estimated by measuring oxygen consumption. The microbial biosensor was used for the measurement of sulfuric acid in a batch culture medium contaminated by microorganisms. A linear relationship between the microbial sensor response and the concentration of sulfuric acid was observed. The response time of biosensor was 5 min and was dependent on the immobilized cell loading of Pseudomonas sp., pH, temperature and corrosive environments. The microbial biosensor response was stable, reproducible and specific for sensing of sulfur oxidizing bacterial activity. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：995 / 1000

页数：6

共 25 条

[1] THIOBACILLUS-NEOPOLITANUS IMPLICATED IN THE DEGRADATION OF CONCRETE TANKS USED FOR POTABLE WATER STORAGE [J].