The effects of hydrostatic pressure on the conformation of plasminogen

被引：14
作者：
Kornblatt, JA
Kornblatt, MJ
Clery, C
Balny, C
机构：
[1] Concordia Univ, Dept Chem & Biochem, Enzyme Res Grp, Montreal, PQ H3G 1M8, Canada
[2] Concordia Univ, Dept Biol, Montreal, PQ H3G 1M8, Canada
[3] Serv Sante Armee, Res Ctr, Unite Biochim, La Tronche, France
[4] INSERM, U128, Montpellier, France
来源：
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1999年 / 265卷 / 01期
关键词：
conformational changes; hydrostatic pressure; plasminogen;
D O I：
10.1046/j.1432-1327.1999.00695.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Plasminogen undergoes a large conformational change when it binds 6-aminohexanoate. Using ultraviolet absorption spectroscopy and native PAGE, we show that hydrostatic pressure brings about the same conformational change. The volume change for this conformational change is -33 mL.mol(-1). Binding of ligand and hydrostatic pressure both cause the protein to open up to expose surfaces that had previously been buried in the interior.
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页码：120 / 126
页数：7
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