Immunoglobulin with High-Titer In Vitro Cross-Neutralizing Hepatitis C Virus Antibodies Passively Protects Chimpanzees from Homologous, but Not Heterologous, Challenge

被引:41
作者
Bukh, Jens [1 ,2 ,3 ,4 ]
Engle, Ronald E. [4 ]
Faulk, Kristina [4 ]
Wang, Richard Y. [5 ]
Farci, Patrizia [4 ]
Alter, Harvey J. [5 ]
Purcell, Robert H. [4 ]
机构
[1] Univ Copenhagen, Dept Infect Dis, Copenhagen Hepatitis Program CO HEP C, Copenhagen, Denmark
[2] Univ Copenhagen, Hvidovre Hosp, Clin Res Ctr, Copenhagen, Denmark
[3] Univ Copenhagen, Fac Hlth & Med Sci, Dept Immunol & Microbiol, Copenhagen, Denmark
[4] NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA
[5] NIH, Warren Grant Magnuson Clin Ctr, Dept Transfus Med, Bethesda, MD 20892 USA
关键词
CELL-CULTURE SYSTEMS; MOLECULAR CLONE; GENOTYPE; 5A; INFECTION; VACCINE; LIVER; GLYCOPROTEIN; PREVENTION; ASSAY; HCV;
D O I
10.1128/JVI.01194-15
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The importance of neutralizing antibodies (NAbs) in protection against hepatitis C virus (HCV) remains controversial. We infused a chimpanzee with H06 immunoglobulin from a genotype 1a HCV-infected patient and challenged with genotype strains efficiently neutralized by H06 in vitro. Genotype 1a NAbs afforded no protection against genotype 4a or 5a. Protection against homologous 1a lasted 18 weeks, but infection emerged when NAb titers waned. However, 6a infection was prevented. The differential in vivo neutralization patterns have implications for HCV vaccine development.
引用
收藏
页码:9128 / 9132
页数:5
相关论文
共 54 条
  • [1] Recombinant Hepatitis C Virus Envelope Glycoprotein Vaccine Elicits Antibodies Targeting Multiple Epitopes on the Envelope Glycoproteins Associated with Broad Cross-Neutralization
    Alexander, Jason
    Wong, Ji-Xhin
    Bhat, Rakesh
    Hockman, Darren
    Logan, Michael
    Chen, Chao
    Levin, Aviad
    Frey, Sharon E.
    Belshe, Robert B.
    Tyrrell, D. Lorne
    Law, John Lok Man
    Houghton, Michael
    [J]. JOURNAL OF VIROLOGY, 2014, 88 (24) : 14278 - 14288
  • [2] Recovery, persistence, and sequelae in hepatitis C virus infection: A perspective on long-term outcome
    Alter, HJ
    Seeff, LB
    [J]. SEMINARS IN LIVER DISEASE, 2000, 20 (01) : 17 - 35
  • [3] The past, present and future of neutralizing antibodies for hepatitis C virus
    Ball, Jonathan K.
    Tarr, Alexander W.
    McKeating, Jane A.
    [J]. ANTIVIRAL RESEARCH, 2014, 105 : 100 - 111
  • [4] In vitro assay for neutralizing antibody to hepatitis C virus:: Evidence for broadly conserved neutralization epitopes
    Bartosch, B
    Bukh, J
    Meunier, JC
    Granier, C
    Engle, RE
    Blackwelder, WC
    Emerson, SU
    Cosset, FL
    Purcell, RH
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (24) : 14199 - 14204
  • [5] Experimental infection of chimpanzees with hepatitis C virus of genotype 5a: Genetic analysis of the virus and generation of a standardized challenge pool
    Bukh, J
    Apgar, CL
    Engle, R
    Govindarajan, S
    Hegerich, PA
    Tellier, R
    Wong, DC
    Elkins, R
    Kew, MC
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1998, 178 (04) : 1193 - 1197
  • [6] HEPATITIS-C VIRUS-RNA IN SOUTHERN AFRICAN BLACKS WITH HEPATOCELLULAR-CARCINOMA
    BUKH, J
    MILLER, RH
    KEW, MC
    PURCELL, RH
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (05) : 1848 - 1851
  • [7] Studies of hepatitis C virus in chimpanzees and their importance for vaccine development
    Bukh, J
    Forns, X
    Emerson, SU
    Purcell, RH
    [J]. INTERVIROLOGY, 2001, 44 (2-3) : 132 - 142
  • [8] Animal Models for the Study of Hepatitis C Virus Infection and Related Liver Disease
    Bukh, Jens
    [J]. GASTROENTEROLOGY, 2012, 142 (06) : 1279 - +
  • [9] Challenge Pools of Hepatitis C Virus Genotypes 1-6 Prototype Strains: Replication Fitness and Pathogenicity in Chimpanzees and Human Liver-Chimeric Mouse Models
    Bukh, Jens
    Meuleman, Philip
    Tellier, Raymond
    Engle, Ronald E.
    Feinstone, Stephen M.
    Eder, Gerald
    Satterfield, William C.
    Govindarajan, Sugantha
    Krawczynski, Krzysztof
    Miller, Roger H.
    Leroux-Roels, Geert
    Purcell, Robert H.
    [J]. JOURNAL OF INFECTIOUS DISEASES, 2010, 201 (09) : 1381 - 1389
  • [10] Hepatitis C virus subtyping by a core-envelope 1-based reverse transcriptase PCR assay with sequencing and its use in determining subtype distribution among Danish patients
    Corbet, S
    Bukh, J
    Heinsen, A
    Fomsgaard, A
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (03) : 1091 - 1100