Immunostimulatory DNA sequences necessary for effective intradermal gene immunization

被引：894

作者：

Sato, Y

Roman, M

Tighe, H

Lee, D

Corr, M

Nguyen, MD

Silverman, GJ

Lotz, M

Carson, DA

Raz, E

机构：

[1] UNIV CALIF SAN DIEGO,DEPT MED,LA JOLLA,CA 92093

[2] UNIV CALIF SAN DIEGO,SAM & ROSE STEIN INST RES AGING,LA JOLLA,CA 92093

来源：

SCIENCE | 1996年 / 273卷 / 5273期

关键词：

D O I：

10.1126/science.273.5273.352

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Vaccination with naked DNA elicits cellular and humoral immune responses that have a T helper cell type 1 bias. However, plasmid vectors expressing large amounts of gene product do not necessarily induce immune responses to the encoded antigens. instead, the immunogenicity of plasmid DNA (pDNA) requires short immunostimulatory DNA sequences (ISS) that contain a CpG dinucleotide in a particular base context. Human monocytes transfected with pDNA or double-stranded oligonucleotides containing the ISS, but not those transfected with ISS-deficient pDNA or oligonucleotides, transcribed large amounts of interferon-alpha, interferon-beta, and interleukin-12. Although ISS are necessary for gene vaccination, they down-regulate gene expression and thus may interfere with gene replacement therapy by inducing proinflammatory cytokines.

引用

页码：352 / 354

页数：3

共 27 条

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