Citrus Flavanones Affect Hepatic Fatty Acid Oxidation in Rats by Acting as Prooxidant Agents

被引:17
作者
Constantin, Rodrigo Polimeni [1 ]
do Nascimento, Gilson Soares [1 ]
Constantin, Renato Polimeni [1 ]
Salgueiro, Clairce Luzia [2 ]
Bracht, Adelar [1 ]
Ishii-Iwamoto, Emy Luiza [1 ]
Yamamoto, Nair Seiko [1 ]
Constantin, Jorgete [1 ]
机构
[1] Univ Maringa, Lab Liver Metab, Dept Biochem, BR-87020900 Maringa, Parana, Brazil
[2] Univ Maringa, Dept Physiol Sci, BR-87020900 Maringa, Parana, Brazil
关键词
MITOCHONDRIAL BETA-OXIDATION; MICROVESICULAR STEATOSIS; RESPIRATORY-CHAIN; FREE-RADICALS; METABOLISM; ANTIOXIDANT; FLAVONOIDS; LIVER; QUERCETIN; FISETIN;
D O I
10.1155/2013/342973
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Citrus flavonoids have a wide range of biological activities and positive health effects on mammalian cells because of their antioxidant properties. However, they also act as prooxidants and thus may interfere with metabolic pathways. The purpose of this work was to evaluate the effects of three citrus flavanones, hesperidin, hesperetin, and naringenin, on several parameters linked to fatty acid oxidation in mitochondria, peroxisomes, and perfused livers of rats. When exogenous octanoate was used as substrate, hesperetin and naringenin reduced the mitochondrial NADH/NAD(+) ratio and stimulated the citric acid cycle without significant changes on oxygen uptake or ketogenesis. When fatty acid oxidation from endogenous sources was evaluated, hesperetin and naringenin strongly reduced the mitochondrial NADH/NAD(+) ratio. They also inhibited both oxygen uptake and ketogenesis and stimulated the citric acid cycle. Hesperidin, on the other hand, had little to no effect on these parameters. These results confirm the hypothesis that citrus flavanones are able to induce a more oxidised state in liver cells, altering parameters related to hepatic fatty acid oxidation. The prooxidant effect is most likely a consequence of the ability of these substances to oxidise NADH upon production of phenoxyl radicals in the presence of peroxidases and hydrogen peroxide.
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页数:12
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