Cotton GhMKK1 Induces the Tolerance of Salt and Drought Stress, and Mediates Defence Responses to Pathogen Infection in Transgenic Nicotiana benthamiana

被引：80
作者：
Lu, Wenjing [1 ]
Chu, Xiaoqian [1 ]
Li, Yuzhen [1 ]
Wang, Chen [1 ]
Guo, Xingqi [1 ]
机构：
[1] Shandong Agr Univ, State Key Lab Crop Biol, Coll Life Sci, Tai An, Shandong, Peoples R China
来源：
PLOS ONE | 2013年 / 8卷 / 07期
基金：
美国国家科学基金会;
关键词：
PROTEIN-KINASE KINASE; AFFECTS DISEASE RESISTANCE; MAP KINASE; OXIDATIVE STRESS; CELL-DEATH; H2O2; PRODUCTION; ARABIDOPSIS; PATHWAY; GENE; ACTIVATION;
D O I：
10.1371/journal.pone.0068503
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Mitogen-activated protein kinase kinases (MAPKK) mediate a variety of stress responses in plants. So far little is known on the functional role of MAPKKs in cotton. In the present study, Gossypium hirsutum MKK1 (GhMKK1) function was investigated. GhMKK1 protein may activate its specific targets in both the nucleus and cytoplasm. Treatments with salt, drought, and H2O2 induced the expression of GhMKK1 and increased the activity of GhMKK1, while overexpression of GhMKK1 in Nicotiana benthamiana enhanced its tolerance to salt and drought stresses as determined by many physiological data. Additionally, GhMKK1 activity was found to up-regulate pathogen-associated biotic stress, and overexpression of GhMKK1 increased the susceptibility of the transgenic plants to the pathogen Ralstonia solanacearum by reducing the expression of PR genes. Moreover, GhMKK1-overexpressing plants also exhibited an enhanced reactive oxygen species scavenging capability and markedly elevated activities of several antioxidant enzymes. These results indicate that GhMKK1 is involved in plants defence responses and provide new data to further analyze the function of plant MAPK pathways.
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页数：14
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