Development of an Oligopeptide Functionalized Surface Plasmon Resonance Biosensor for Online Detection of Glyphosate

被引：48

作者：

Ding, Xiaokang ^{[1
]}

Yang, Kun-Lin ^{[1
]}

机构：

[1] Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore 117576, Singapore

来源：

ANALYTICAL CHEMISTRY | 2013年 / 85卷 / 12期

关键词：

AMINOMETHYLPHOSPHONIC ACID; CAPILLARY-ELECTROPHORESIS; COULOMETRIC DETECTION; HERBICIDE GLYPHOSATE; 3-PHOSPHATE SYNTHASE; SELECTIVE DETECTION; MASS-SPECTROMETRY; HUMAN SERUM; WATER; CHROMATOGRAPHY;

D O I：

10.1021/ac400273g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report a surface plasmon resonance (SPR) biosensor for online detection of glyphosate. The surface of the sensing element is decorated with an oligopeptide, TPFDLRPSSDTR, which is identified by using phage display library. This oligopeptide shows high binding specificity for glyphosate (K-D = 8.6 mu M), probably because of the presence of R and D in the oligopeptide. To detect glyphosate in buffer solution, an SPR gold sensor chip is modified by using the oligopeptide with a surface density of 0.6 1/nm(2). The sensitivity of this oligopeptide-functionalized SPR biosensor is 1.02 RU/mu M whereas the limit of detection (LOD) is 0.58 mu M. This oligopeptide-functionalized SPR biosensor also shows good specificity against other analytes such as glycine, thiacloprid, and imidacloprid.

引用

页码：5727 / 5733

页数：7

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