Liquid chromatographic analysis of supercritical carbon dioxide extracts of Schizandra chinensis

被引：24
作者：
Bártlová, M
Opletal, L
Chobot, V
Sovová, H
机构：
[1] Acad Sci Czech Republ, Inst Chem Proc Fundamentals, Prague 16502 6, Czech Republic
[2] Charles Univ Prague, Fac Pharm Hradec Kralove, Hradec Kralove 50002, Czech Republic
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2002年 / 770卷 / 1-2期
关键词：
Schizandra chinensis; carbon dioxide;
D O I：
10.1016/S1570-0232(01)00634-1
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Six major lignans (schizandrin, gomisin A, deoxyschizandrin, gamma-schizandrin, gomisin N, wuweizisu C) in the caulomas and leaves of Schizandra chinensis (Turcz.) Baill., and cinnamic acid in the leaves of the plant, were quantitatively analysed by high-performance liquid chromatography in reversed-phase mode with UV detection. Resolution of the determined lignans was evaluated for two multistep gradients applied. Samples for HPLC analysis were prepared by extraction with supercritical carbon dioxide at pressures of 20-27 MPa and temperatures of 40-60 degreesC. Kinetics of the extraction of individual components was measured and simulated with a model. (C) 2002 Elsevier Science B.V. All rights reserved.
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收藏
页码：283 / 289
页数：7
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