Synthetic peptides identify a second periplasmic site for the plug of the SecYEG protein translocation complex

被引：9

作者：

Robson, Alice ^{[1
]}

Carr, Beatrice ^{[1
]}

Sessions, Richard B. ^{[1
]}

Collinson, Ian ^{[1
]}

机构：

[1] Univ Bristol, Dept Biochem, Bristol BS8 1TD, Avon, England

来源：

FEBS LETTERS | 2009年 / 583卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Membrane proteins; Protein translocation; SecYEG; Synthetic peptides; Molecular modelling; PROJECTION STRUCTURE; CHANNEL; DOMAIN; SECA;

D O I：

10.1016/j.febslet.2008.12.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A short helix in the centre of the SecY subunit serves as a 'plug' blocking the protein channel. This site must be vacated if the channel is to open and accommodate translocating protein. We have synthesised a peptide mimic of this plug, and show that it binds to E. coli SecYEG, identifying a distinct and peripheral binding site. We propose that during active translocation the plug moves to this second discrete site and chart its position. Deletion of the plug in SecY increases the stoichiometry of the peptide-SecYEG interaction by also exposing the location it occupies in the channel. Binding of the plug peptide to the channel is unaffected by SecA. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：207 / 212

页数：6

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