From surface self-assembly to crystallization: Prediction of protein crystallization conditions

被引:26
作者
Jia, YW [1 ]
Liu, XY [1 ]
机构
[1] Natl Univ Singapore, Dept Phys, Singapore 117542, Singapore
关键词
D O I
10.1021/jp0536089
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
A new criterion based on surface and volume diffusion kinetics was established to predict protein crystallization. Similar to the layer-by-layer crystal growth process of protein, the kinetics of the two-dimensional self-assembly of protein at the aqueous solution surface provides a convenient and reliable way to estimate the surface integration and the volume transport during protein crystallization. Both the surface and diffusion kinetics were estimated based on the protein self-assembly at the air/solution interface, which can be obtained by measuring the surface tension. A crystallization coefficient is found to provide an effective and reliable criterion to predict protein crystallization conditions. This criterion has been applied to lysozyme, concanavalin A and BSA crystallization, and it turns out to be very successful and more reliable than the second virial coefficient criterion.
引用
收藏
页码:6949 / 6955
页数:7
相关论文
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