13C-metabolic flux analysis of lipid accumulation in the oleaginous fungus Mucor circinelloides

被引：45

作者：

Zhao, Lina ^{[1
]}

Zhang, Huaiyuan ^{[1
]}

Wang, Liping ^{[1
]}

Chen, Haiqin ^{[1
,2
]}

Chen, Yong Q. ^{[1
,2
]}

Chen, Wei ^{[1
,2
]}

Song, Yuanda ^{[1
]}

机构：

[1] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China

[2] Synergist Innovat Ctr Food Safety & Nutr, Wuxi 214122, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2015年 / 197卷

基金：

中国国家自然科学基金; 国家高技术研究发展计划(863计划);

关键词：

Metabolic flux analysis; Mucor circinelloides; Lipid accumulation; Pentose phosphate pathway; POLYUNSATURATED FATTY-ACIDS; YEAST YARROWIA-LIPOLYTICA; MALIC ENZYME; DOCOSAHEXAENOIC ACID; EFFICIENT PRODUCTION; NADPH; MICROORGANISMS; METABOLISM; EXPRESSION; INCREASE;

D O I：

10.1016/j.biortech.2015.08.035

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The oleaginous fungus Mucor circinelloides is of industrial interest because it can produce high levels of polyunsaturated fatty acid gamma-linolenic acid. M. circinelloides CBS 277.49 is able to accumulate less than 15% of cell dry weight as lipids, while M. circinelloides WJ11 can accumulate lipid up to 36%. In order to better understand the mechanisms behind the differential lipid accumulation in these two strains, tracer experiments with C-13-glucose were performed with the growth of M. circinelloides and subsequent gas chromatography-mass spectrometric detection of C-13-patterns in proteinogenic amino acids was carried out to identify the metabolic network topology and estimate intracellular fluxes. Our results showed that the high oleaginous strain WJ11 had higher flux of pentose phosphate pathway and malic enzyme, lower flux in tricarboxylic acid cycle, higher flux in glyoxylate cycle and ATP: citrate lyase, together, it might provide more NADPH and substrate acetyl-CoA for fatty acid synthesis. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：23 / 29

页数：7

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