Pretreatment Effect of Running Exercise on HSP70 and DOX-Induced Cardiotoxicity

Objective: The purpose of this study was to determine pretreatment effects of moderate-term endurance training before the various dosages (10 and 20(mg.kg)(-1)) of DOX on a heat shock protein (HSP70(kda)) and cardiotoxicity in heart tissue. Methods: Forty-eight male rats were randomly assigned to nontraining (NT) and training (T) groups and three subgroups; DOX10(mg.kg)(-1) and DOX20(mg.kg)(-1) and saline treatment. The training program included treadmill running between 25-39 min/day and 15-17 m/min, 5 days/wk for 3 wk. Result: DOX administration, in particularly with 20(mg.kg)(-1), caused up-regulation of oxidants and cardiac damage (MDA, CK, CPK-MB and CK/CPK-MB) and down-regulation of cardioprotection (HSP70, SOD) markers, as compared to NT+saline group. Pretreatment effect of treadmill running endurance exercise in the presence of DOX with 10(mg.kg)(-1) caused a significant increase in HSP70, SOD and a significant decrease in MDA and insignificant decrease in CK, CPK-MB and CK/CPK-MB, in comparison T+DOX10 with NT+DOX10 group. However, there was no significant difference between T+DOX10mg.kg-1 and T+DOX20(mg.kg)(-1) in the aforesaid markers. Conclusion: Dox-induced cardiotoxicity is related to oxidative stress. Our study suggests that pretreatment with endurance exercise may be considered as a potentially useful strategy to improve myocardial tolerance against single dose DOX-induced oxidative damage.