Transportin-SR, a nuclear import receptor for SR proteins

被引：188

作者：

Kataoka, N ^{[1
]}

Bachorik, JL ^{[1
]}

Dreyfuss, G ^{[1
]}

机构：

[1] Univ Penn, Sch Med, Howard Hughes Med Inst, Dept Biochem & Biophys, Philadelphia, PA 19104 USA

来源：

JOURNAL OF CELL BIOLOGY | 1999年 / 145卷 / 06期

关键词：

SR proteins; RS domain; nuclear localization signal; nuclear import receptor; RanGTP;

D O I：

10.1083/jcb.145.6.1145

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The SR proteins, a group of abundant arginine/serine (RS)-rich proteins, are essential pre-mRNA splicing factors that are localized in the nucleus,The RS domain of these proteins serves as a nuclear localization signal. We found that RS domain-bearing proteins do not utilize any of the known nuclear import receptors and identified a novel nuclear import receptor specific for SR proteins. The SR protein import receptor. termed transportin-SR (TRN-SR), binds specifically and directly to the RS domains of ASF/SF2 and SC35 as well as several other SR proteins. The nuclear transport regulator RanGTP abolishes this interaction. Recombinant TRN-SR mediates nuclear import of RS domain-bearing proteins in vitro. TRN-SR has amino acid sequence similarity to several members of the importin beta/transportin family. These findings strongly suggest that TRN-SR is a nuclear import receptor for the SR protein family.

引用

页码：1145 / 1152

页数：8

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