Comparison of human articular chondrocyte and chondroprogenitor cocultures and monocultures: To assess chondrogenic potential and markers of hypertrophy

被引:15
作者
Vinod, Elizabeth [1 ,4 ]
Kachroo, Upasana [1 ]
Ozbey, Ozlem [2 ]
Sathishkumar, Solomon [1 ]
Boopalan, P. R. J. V. C. [3 ,4 ]
机构
[1] Christian Med Coll & Hosp, Dept Physiol, Vellore 632002, Tamil Nadu, India
[2] Akdeniz Univ, Sch Med, Dept Histol & Embryol Campus, TR-07070 Antalya, Turkey
[3] Christian Med Coll & Hosp, Dept Orthopaed, Vellore 632004, Tamil Nadu, India
[4] Christian Med Coll & Hosp, Ctr Stem Cell Res, Vellore 632002, Tamil Nadu, India
关键词
Cartilage; Chondrocytes; Chondrogenesis; Chondroprogenitors; Co-culture; MESENCHYMAL STEM-CELLS; PROGENITOR CELLS; GENE-EXPRESSION; STROMAL CELLS; BONE-MARROW; OSTEOARTHRITIC CARTILAGE; MONOLAYER-CULTURE; FAT PAD; TISSUE; SURFACE;
D O I
10.1016/j.tice.2019.01.007
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Background and Objective: In the field of cartilage repair, use of two or more cell populations such as mesenchymal stem cells with chondrocytes in an in-vitro co-culture synergistic environment has been attempted to evade limitations of monoculture systems and promote/induce chondrogenesis. Articular cartilage-derived chondroprogenitors (CPs), considered to have stem-cell like characteristics have been proposed as a potential contender for neocartilage development. Our objective was to assess whether co-cultures using different ratios of chondrocytes(C) and CPs would demonstrate better results in terms of growth kinetics, surface marker expression, chondrogenic potential, tendency for hypertrophy and glycosaminoglycan deposition than monocultures. Study design: Human chondrocytes and CPs (fibronectin adhesion assay) from the same cartilage source were isolated. Passage 2 cells were subjected to monolayer/pellet cultures and were grown as monocultures and cocultures at the following percentage ratios(C:CP) 80:20, 65:35, 50:50, 35:65 and 20:80. Results: Analysis of data acquired from population doubling, flow cytometry, RT-PCR and Safranin O uptake demonstrated similar results in all monoculture and co-culture groups with no significant inter-group variation, even when reported specific markers of identification (CD54 and CD44: chondrocyte markers) and isolation (CD29 and CD49e: forming heterodimeric fibronectin receptor for CP sorting) were examined. Conclusion: In conclusion, this study suggests the need for improved sorting techniques based on a characteristic differentiating biomarker for selection of cells which are true representatives of CPs possessing properties of enhanced chondrogenesis and reduced hypertrophy.
引用
收藏
页码:42 / 48
页数:7
相关论文
共 39 条
[1]   Matrix-induced autologous mesenchymal stem cell implantation versus matrix-induced autologous chondrocyte implantation in the treatment of chondral defects of the knee: a 2-year randomized study [J].
Akgun, Isik ;
Unlu, Mehmet C. ;
Erdal, Ozan A. ;
Ogut, Tahir ;
Erturk, Murat ;
Ovali, Ercument ;
Kantarci, Fatih ;
Caliskan, Gurkan ;
Akgun, Yamac .
ARCHIVES OF ORTHOPAEDIC AND TRAUMA SURGERY, 2015, 135 (02) :251-263
[2]  
[Anonymous], 2009, SPORTS HLTH, DOI DOI 10.1177/1941738109350438
[3]   Co-culture of infrapatellar fat pad-derived mesenchymal stromal cells and articular chondrocytes in plasma clot for cartilage tissue engineering [J].
Arora, Aditya ;
Sriram, M. ;
Kothari, Anjaney ;
Katti, Dhirendra S. .
CYTOTHERAPY, 2017, 19 (07) :881-894
[4]  
Bian L, 2011, TISSUE ENG PT A, V17, P1137, DOI [10.1089/ten.tea.2010.0531, 10.1089/ten.TEA.2010.0531]
[5]   TREATMENT OF DEEP CARTILAGE DEFECTS IN THE KNEE WITH AUTOLOGOUS CHONDROCYTE TRANSPLANTATION [J].
BRITTBERG, M ;
LINDAHL, A ;
NILSSON, A ;
OHLSSON, C ;
ISAKSSON, O ;
PETERSON, L .
NEW ENGLAND JOURNAL OF MEDICINE, 1994, 331 (14) :889-895
[6]   The MSC: An Injury Drugstore [J].
Caplan, Arnold I. ;
Correa, Diego .
CELL STEM CELL, 2011, 9 (01) :11-15
[7]   Co-culture of chondrocytes and bone marrow mesenchymal stem cells in vitro enhances the expression of cartilaginous extracellular matrix components [J].
Chang, Qing ;
Cui, Wei-ding ;
Fan, Wei-min .
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, 2011, 44 (04) :303-310
[8]   Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather than amplification of progenitor cells [J].
Diaz-Romero, Jose ;
Nesic, Dobrila ;
Grogan, Shawn Patrick ;
Hein, Paul ;
Mainil-Varlet, Pierre .
JOURNAL OF CELLULAR PHYSIOLOGY, 2008, 214 (01) :75-83
[9]   Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement [J].
Dominici, M. ;
Le Blanc, K. ;
Mueller, I. ;
Slaper-Cortenbach, I. ;
Marini, F. C. ;
Krause, D. S. ;
Deans, R. J. ;
Keating, A. ;
Prockop, D. J. ;
Horwitz, E. M. .
CYTOTHERAPY, 2006, 8 (04) :315-317
[10]   The surface of articular cartilage contains a progenitor cell population [J].
Dowthwaite, GP ;
Bishop, JC ;
Redman, SN ;
Khan, IM ;
Rooney, P ;
Evans, DJR ;
Haughton, L ;
Bayram, Z ;
Boyer, S ;
Thomson, B ;
Wolfe, MS ;
Archer, CW .
JOURNAL OF CELL SCIENCE, 2004, 117 (06) :889-897