Comparison of the structural and kinetic properties of the cytochrome c nitrite reductases from Escherichia coli, Wolinella succinogenes, Sulfurospirillum deleyianum and Desulfolvibrio desulfuricans

被引：20
作者：
Clarke, TA
Hemmings, AM
Burlat, B
Butt, JN
Cole, JA
Richardson, DJ [1 ]
机构：
[1] Univ E Anglia, Sch Biol Sci, Ctr Met Prot Spect & Biol, Norwich NR4 7TJ, Norfolk, England
[2] Univ E Anglia, Sch Chem Sci & Pharm, Ctr Met Prot Spect & Biol, Norwich NR4 7TJ, Norfolk, England
[3] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England
来源：
BIOCHEMICAL SOCIETY TRANSACTIONS | 2006年 / 34卷
基金：
英国生物技术与生命科学研究理事会;
关键词：
active-site channel; cytochrome c; nitrite reductase; NrfA; sequence alignment; structural alignment;
D O I：
10.1042/BST0340143
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The recent crystallographic characterization of NrfAs from Sulfurospirillum, deleyianum, Wolinella succinogenes, Escherichia coli and Desulfovibrio desulfuricans allows structurally conserved regions to be identified. Comparison of nitrite and sulphite reductase activities from different bacteria shows that the relative activities vary according to organism. By comparison of both amino acid sequences and structures, differences can be identified in the monomer-monomer interface and the active-site channel; these differences could be responsible for the observed variance in substrate activity and indicate that subtle changes in the NrfA structure may optimize the enzyme for different roles.
引用
收藏
页码：143 / 145
页数：3
相关论文
共 9 条
[1]   Structure and spectroscopy of the periplasmic cytochrome c nitrite reductase from Escherichia coli [J].
Bamford, VA ;
Angove, HC ;
Seward, HE ;
Thomson, AJ ;
Cole, JA ;
Butt, JN ;
Hemmings, AM ;
Richardson, DJ .
BIOCHEMISTRY, 2002, 41 (09) :2921-2931
[2]   Cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774 -: The relevance of the two calcium sites in the structure of the catalytic subunit (NrfA) [J].
Cunha, CA ;
Macieira, S ;
Dias, JM ;
Almeida, G ;
Gonçalves, LL ;
Costa, C ;
Lampreia, J ;
Huber, R ;
Moura, JJG ;
Moura, I ;
Romao, MJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (19) :17455-17465
[3]   Cytochrome c nitrite reductase from Wolinella succinogenes -: Structure at 1.6 Å resolution, inhibitor binding, and heme-packing motifs [J].
Einsle, O ;
Stach, P ;
Messerschmidt, A ;
Simon, J ;
Kröger, A ;
Huber, R ;
Kroneck, PMH .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (50) :39608-39616
[4]   Structure of cytochrome c nitrite reductase [J].
Einsle, O ;
Messerschmidt, A ;
Stach, P ;
Bourenkov, GP ;
Bartunik, HD ;
Huber, R ;
Kroneck, PMH .
NATURE, 1999, 400 (6743) :476-480
[5]   Characterization of a heme c nitrite reductase from a non-ammonifying microorganism, Desulfovibrio vulgaris Hildenborough [J].
Pereira, IAC ;
LeGall, J ;
Xavier, AV ;
Teixeira, M .
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2000, 1481 (01) :119-130
[6]   Respiratory detoxification of nitric oxide by the cytochrome c nitrite reductase of Escherichia coli [J].
Poock, SR ;
Leach, ER ;
Moir, JWB ;
Cole, JA ;
Richardson, DJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (26) :23664-23669
[7]   Enzymology and bioenergetics of respiratory nitrite ammonification [J].
Simon, J .
FEMS MICROBIOLOGY REVIEWS, 2002, 26 (03) :285-309
[8]   Bacterial cytochrome c nitrite reductase:: new structural and functional aspects [J].
Stach, P ;
Einsle, O ;
Schumacher, W ;
Kurun, E ;
Kroneck, PMH .
JOURNAL OF INORGANIC BIOCHEMISTRY, 2000, 79 (1-4) :381-385
[9]   MOLECULAR-PROPERTIES OF THE DISSIMILATORY SULFITE REDUCTASE FROM DESULFOVIBRIO-DESULFURICANS (ESSEX) AND COMPARISON WITH THE ENZYME FROM DESULFOVIBRIO-VULGARIS (HILDENBOROUGH) [J].
STEUBER, J ;
ARENDSEN, AF ;
HAGEN, WR ;
KRONECK, PMH .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1995, 233 (03) :873-879
1